Nucleic acid to activate gene expression and protein production

A method for increasing the expression of a protein of interest in a host cell comprising the steps of:

• a) synthesizing a nucleic acid for increasing the expression of a protein of interest comprising at least one copy of the 28-oligonucleotide with the base sequence of SEQ ID NO:
• 1 and preparing compositions comprising said nucleic acid, a buffer, a divalent ion chelating agent, and combinations thereof;
• b) contacting the nucleic acid composition with a linearized expression vector encoding for a protein of interest and ligating the nucleic acid downstream of the gene coding sequence, for transcription at the 3′ untranslated region (3′UTR) through incubation with a DNA Ligase in the appropriate buffer and temperature conditions, so as to obtain an expression vector comprising at least one copy of the nucleic acid with SEQ ID NO:
• 1 downstream of the gene of interest coding region;
• c) introducing the said expression vectors or the nucleic acid for increasing the expression of a protein or interest comprising at least one copy of the 28-oligonucleotide with the base sequence of SEQ ID NO:
• 1 into a host cell, trough transient or stable transformation, transduction, transfection or through gene editing protocols, wherein said nucleic acid is introduced into the host cell downstream of the gene of interest coding region, so as to produce a host cell such as the host cell comprising at least one exogenous copy of the nucleic acid for increasing the expression of a protein of interest comprising at least one copy of the 28-oligonucleotide with the base sequence of SEQ ID NO:
• 1, introduced through gene editing;
• and
• d) expressing the protein of interest.