High-affinity t cell receptor against prame

A T cell receptor (TCR), which has an activity of binding to VLDGLDVLL-HLA-A0201 complex, the T cell receptor comprises a TCR α chain variable domain and a TCR β chain variable domain, the α chain variable domain of the TCR comprises an amino acid sequence having at least 90% of sequence homology with the amino acid sequence shown in SEQ ID NO:

• 1, and the β chain variable domain of the TCR comprises an amino acid sequence having at least 90% of sequence homology with the amino acid sequence shown in SEQ ID NO:
• 2, the TCRα chain variable domain comprises three CDR regions, and the reference sequences of the three CDR regions of the TCR α chain variable domain are listed as follows,
• CDR1α:
• DRGSQS
• CDR2α:
• IYSNGD
• CDR3α:
• AVARTYTGNQFY,
• the TCR β chain variable domain comprises three CDR regions, and the reference sequences of the three CDR regions of the TCR β chain variable domain are listed as follows,
• CDR1β:
• SEHNR
• CDR2β:
• FQNEAQ
• CDR3β:
• ASSSQKFSGIQPQH,
• and the TCRα chain variable domain contains at least one of the following mutations:
• Residue before mutation
• Residue after mutation
• S at position 4 of CDR1α
• T or A
• S at position 6 of CDR1α
• A
• I at position 1 of CDR2α
• Q or L or T
• Y at position 2 of CDR2α
• V
• S at position 3 of CDR2α
• M or V or Q
• N at position 4 of CDR2α
• P or D
• A at position 3 of CDR3α
• V
• R at position 4 of CDR3α
• L
• T at position 5 of CDR3α
• S
• Y at position 6 of CDR3α
• W
• T at position 7 of CDR3α
• K or A or R or L or Q or F
• G at position 8 of CDR3α
• S
• N at position 9 of CDR3α
• T
• Q at position 10 of CDR3α
• G or R
• and/or, the TCR β chain variable domain contains at least one of the following mutations:
• Residue before mutation
• Residue after mutation
• N at position 3 of CDR2β
• D or G
• E at position 4 of CDR2β
• S or R
• A at position 5 of CDR2β
• I or S
• Q at position 6 of CDR2β
• E
• S at position 3 of CDR3β
• N
• S at position 4 of CDR3β
• A or P or N or K or Q or T or M or R
• Q at position 5 of CDR3β
• S or G or T
• K at position 6 of CDR3β
• P or G or L
• F at position 7 of CDR3β
• V or L.

A TCR, wherein the TCR is selected from the group consisting of:

• Sequence of α chain variable
• Sequence of β chain variable
• TCR No.
• domain SEQ ID NO:
• domain SEQ ID NO:
• s-1
• 9
• 4
• s-2
• 10
• 4
• s-3
• 11
• 4
• s-4
• 12
• 4
• s-5
• 13
• 4
• s-6
• 14
• 4
• s-7
• 15
• 4
• s-8
• 3
• 35
• s-9
• 3
• 36
• s-10
• 3
• 37
• s-11
• 3
• 38
• s-12
• 3
• 39
• s-13
• 3
• 40
• s-14
• 3
• 41
• s-15
• 3
• 42
• s-16
• 3
• 43
• s-17
• 3
• 44
• s-18
• 16
• 45
• s-19
• 17
• 46
• s-20
• 18
• 47
• s-21
• 19
• 48
• s-22
• 20
• 49
• s-23
• 21
• 45
• s-24
• 22
• 47
• s-25
• 23
• 50
• s-26
• 24
• 45
• s-27
• 25
• 45
• s-28
• 26
• 51
• s-29
• 27
• 50
• s-30
• 28
• 50
• s-31
• 29
• 52
• s-32
• 30
• 49
• s-33
• 31
• 50
• s-34
• 32
• 45
• s-35
• 33
• 47
• s-36
• 34
• 49.