Over-expression of gds1 in yeast for increased ethanol and decreased acetate production
AssigneeDanisco US Inc.
InventorsZhongqiang CHEN, Min QI, Luan TAO, Quinn Qun ZHU
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Abstract
Described are compositions and methods relating to modified yeast that over-express the glycerol-deficient suppressor, GDS1. The yeast produces an increased amount of ethanol and a deceased amount of acetate compared to parental cells. Such yeast is particularly useful for large-scale ethanol production from starch substrates where acetate in an undesirable end product.
Claims24
Modified yeast cells derived from parental yeast cells, the modified cells comprising a genetic alteration that causes the modified cells to produce an increased amount of GDS1 polypeptides compared to the parental cells, wherein the modified cells produce during fermentation a decreased amount of acetate compared to the amount of acetate produced by the parental cells under identical fermentation conditions.
The modified cells of any of claims 1-5, wherein the cells further comprising one or more genes of the phosphoketolase pathway.
A method for decreasing the production of acetate from yeast cells grown on a carbohydrate substrate, comprising:
- introducing into parental yeast cells a genetic alteration that increases the production of GDS1 polypeptides compared to the amount produced in the parental cells.
A method for increasing the production of ethanol from yeast cells grown on a carbohydrate substrate, comprising:
- introducing into parental yeast cells a genetic alteration that increases the production of GDS1 polypeptides compared to the amount produced in the parental cells.
Dependencies are inferred from the claim text.