Optimized zika virus envelope gene and expression thereof
AssigneeHawaii Biotech, Inc.
InventorDavid E. Clements
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Abstract
The present invention is directed to the expression and secretion the Zika virus envelope protein. Elements of the pre-membrane and envelope sequence have been modified to enhance the expression of the envelope protein as a secreted product in the culture medium of transformed insect cell lines. The expressed and purified product is suitable as a vaccine antigen.
Claims16
1-27. (canceled)
An expression vector comprising a DNA sequence encoding a Zika virus pre-membrane and envelope protein, wherein expression of the DNA sequence results in secretion of a soluble envelope protein in the culture medium.
An expression vector for expression and secretion of heterologous proteins in cultured insect cells, wherein the expression vector comprises the expression cassette shown in SEQ ID NO:7.
A vaccine comprising an effective amount of purified Zika virus envelope protein (E), wherein the protein is secretable into growth medium when expressed recombinantly in a host cell;
- and an effective amount of adjuvant, wherein the vaccine induces the production of neutralizing antibodies in human subjects.
Dependencies are inferred from the claim text.