Proteins and immunizing compositions containing klebsiella proteins and methods of use
AssigneeEpitopix, LLC
InventorsDouglas T. Burkhardt, Daryll Emery, Darren Straub
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Abstract
Provided herein are isolated proteins isolatable from a Klebsiella spp. Also provided are compositions that include one or more of the proteins, and methods for making and methods for using the proteins.
Claims35
A composition comprising:
- at least two isolated proteins having molecular weights of 82 kDa, 78 kDa, 72 kDa, or 68 kDa, wherein molecular weight is determined by electrophoresis on a sodium dodecyl sulfate-polyacrylamide gel, wherein the proteins are isolatable from a Klebsiella pneumoniae when incubated in media comprising an iron chelator and not isolatable when grown in the media without the iron chelator, and wherein the composition protects an animal against infection with K. pneumoniae;
- an adjuvant;
- and
- a pharmaceutically acceptable carrier.
The composition of claim 7 further comprising a protein comprising an amino acid sequence that is structurally similar to, or has 100% identity with, SEQ ID NO:45, SEQ ID NO:46, SEQ ID NO:49, SEQ ID NO:51, SEQ ID NO:52, SEQ ID NO:53, SEQ ID NO:58, SEQ ID NO:61, or SEQ ID NO:64.
A composition comprising:
- at least two proteins that are structurally similar to, or have 100% identity with, a protein selected from SEQ ID NO:41, SEQ ID NO:58, SEQ ID NO:61, and SEQ ID NO:64;
- an adjuvant;
- and
- a pharmaceutically acceptable carrier.
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21-24. (canceled)
The method of claim 23 wherein the gram negative microbe is selected from K. pneumoniae, K. oxytoca, E. coli, Enterobacter spp., Serratia spp., Proteus spp., Citrobacter spp., or a combination thereof.
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Dependencies are inferred from the claim text.