Plant extract obtained from morus plant leaves, compositions containing same, method of extraction and uses thereof
AssigneeBotanic Century (Beijing) Co. Ltd.
InventorsChen Xie, Yingshu Zou
Life of this patent
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Abstract
An extract from leaves of mulberry is disclosed. The extract has an IC50 value sufficient to inhibit a-glucosidase I. The extract may comprise 5-40%(w/w) total imino sugars and 20-70% (w/w) total amino acids. The extract may reduce the production of melanin for the treatment of such ailments or diseases caused by pigmentation as freckle, chloasma, striae gravidarum, sensile plaque and melanoma. The extract may also control blood glucose level.
Claims22
A plant extract obtained from Morus plant leaves which has an IC50 value of less than 90 μg/ml with respect to inhibition of α-glucosidase I, comprising:
- 5-40% (w/w) total imino sugars, as measured by at least one of quantitative High Performance Liquid Chromatography (HPLC) and liquid chromatography/mass spectrometry (LC-MS), the imino sugars including at least one of 1-deoxynojirimycin 031% fagomine and N-methyl-DNJ;
- and
- 20-70% (w/w) total amino acids, wherein the extract is at least one of pale yellow and nearly white and is easily soluble in water.
A method for producing an extract, from Morus plant leaves, which has an IC50 value to inhibit α-glucosidase I at a concentration of less than 90 μg/ml comprising the steps of:
- a. Conducting a water or alcoholic extraction step from leaf material;
- b. Conducting a column chromatography purification step using a strong acidic cation exchange resin, washing with water, and eluting with an ammonia solution collecting the eluent and removing the ammonia therefrom;
- and
- d. Concentrating and drying the extract.
17-19. (canceled)
24-25. (canceled)
Dependencies are inferred from the claim text.