Galloylated procyanidins for increasing intracellular nitric oxide production

A method of treating an endovascular dysfunction, comprising:

• administering a composition to a subject, wherein the composition comprises:
• a a therapeutically effective amount of one or more galloylated procyanidins having between two and five epicatechin monomers;
• wherein the one or more galloylated procyanidins include epicatechin-(4-8)-epicatechin-(4-8)-epicatechin-gallate, catechin trimer gallate, catechin dimer gallate, or combinations thereof;
• and
• a pharmaceutically acceptable excipient or carrier.

A method of extracting or isolating one or more galloylated procyanidins from a raw material that contains polyphenols, catechins, epicatechins, and galloylated epicatechins, the method comprising:

• extracting the polyphenols, catechins, epicatechins, and galloylated epicatechins from the raw material using hot water at a temperature of about 80° C. to about 85° C. to provide an unrefined material;
• passing the unrefined material through a mesh filter to provide a filtered material;
• absorbing the filtered material with a macro-porous absorption resin;
• eluting impurities from the filtered material absorbed to the resin using pure water;
• eluting the material from the resin in ethanol and collecting an ethanol eluent fraction therefrom;
• concentrating the ethanol eluent fraction and recovering a solvent using a vacuum system to provide a resulting material;
• pasteurizing, sterilizing, and cooling the resulting material;
• spray drying the material into a powder;
• and
• sifting and v-blending the powder.

A method of extracting or isolating one or more galloylated procyanidins from a sample, comprising:

• initially extracting polyphenols, catechins, epicatechins, and galloylated epicatechins from the sample using ethyl acetate;
• further extracting the polyphenols, catechins, epicatechins, and galloylated epicatechins from the sample using water to provide a resulting material;
• eluting the resulting material using resin and diluting the material with ethanol;
• filtering the material using activated carbon;
• concentrating the material;
• spray drying the material into a powder;
• and
• v-blending, sieving, and de-ironing the powder.