Apparatus, system, and method for detecting a target substance

An apparatus for detecting the presence of a targeted substance in a liquid, the apparatus comprising:

• a bottom layer comprising a channel;
• a detection layer for receiving a liquid, wherein the detection layer is disposed in the channel, and wherein the detection layer is capable of displaying a signal that a targeted substance is present or absent;
• and
• a top layer disposed over the detection layer and coupled to the detection layer or to the bottom layer, wherein the top layer comprises at least one opening over a portion of the detection layer, and wherein the apparatus comprises a boundary that substantially prevents liquid entrainment in the apparatus.

A method of making an apparatus for detecting the presence of a targeted substance in a liquid medium comprising:

• providing a detection layer configured to detect the presence or absence of a targeted sub stance;
• coupling the detection layer to a bottom layer;
• and
• coupling a top layer over the detection layer,
• wherein the apparatus is capable of being positioned on, within, or below an implement.