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US 2021/0329954 A1Published application
Processes for transglucosylation of steviol glycosides and steviol glycosides compositions prepared by the same
AssigneeBeijing Gingko Group Biological Technology Co., Ltd.
InventorsVaruzhan ABELYAN, Chunhua LI, Yanmei LI, Xiangang LYU, Yang ZHANG, Chunguang DONG
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Abstract
A process for transglucosylation of steviol glycosides. A sweetener composition comprises at least 95% total glycosylated steviol glycosides.
Claims28
A process for producing α-1,4-glucosylated steviol glycosides, said process comprising:
- providing a steviol glycoside and a donor of glucosyl residues, wherein both are mixed in a predetermined ratio to obtain a reaction solution;
- adding CGTase for transglucosylation of steviol glycosides to the reaction solution;
- whereby the reaction solution is incubated at a predetermined temperature for a predetermined period;
- passing the reaction solution through a chromatographic column packed with sufficient amount of specific polar macroporous polymeric adsorbent;
- washing the column to remove impurities;
- and
- eluting adsorbed non-modified steviol glycosides and glycosylated steviol glycosides with an organic solvent solution;
- and
- removing the organic solvent, concentrating, and drying to produce a purified sweetener composition comprising α-1,4-glucosylated steviol glycosides.
6 more elements
A steviol glycosides composition, comprising no less than 95% total glycosylated steviol glycosides on an anhydrous basis, wherein the glycosylated steviol glycosides are represented by the following formula II:
- (formula II)
- wherein R1=α-1,4-(Glu)n=0-4;
- R2=β-1,3-(Glu)n=0-2;
- R3=α-1,4-(Glu)n=0-4;
- R4=β-1,3-(Glu)n=0-2;
- R5=β-1,2-(Glu)n=0-2;
- R6=α-1,4-(Glu)n=0-4;
- R7=β-1,3-(Glu)n=0-2;
- R8=β-1,2-(Glu or Rha)n=0-2;
- wherein C13-G1 is Glu;
- C13-G2 is β-Glu;
- β-Xyl or α-Rha;
- C19-G1 is Glu;
- wherein Glu is glucose residue;
- Xyl is xylose residue;
- Rha is Rhamnose residue.
13 more elements
Dependencies are inferred from the claim text.