Methods for improved poxvirus yields
AssigneeArizona Board of Regents on behalf of Arizona State University
InventorsDouglas Grant MCFADDEN, Mohammed Masmudur RAHMAN
Life of this patent
Estimating the term…
Abstract
A method of producing poxviruses at an increased growth rate and/or progeny virus titer in cells, the method including: contacting a host cell with an effective amount of nucleocytoplasmic transport inhibitor; contacting the host cell with a poxvirus of interest under conditions that permit the poxvirus of interest to adsorb to the surface of the host cell; and culturing the host cell to produce progeny poxvirus of interest.
Claims44
A method for replicating a poxvirus, comprising:
- exposing a host cell to an effective amount of a nucleocytoplasmic transport inhibitor and the poxvirus under conditions that permit the poxvirus to adsorb to the surface of the host cell;
- and
- incubating the infected host cell in a culture medium to allow for replication of the poxvirus.
A method of producing poxviruses at an increased growth rate and/or titer in cells, the method comprising:
- contacting a host cell with an effective amount of a nucleocytoplasmic transport inhibitor;
- contacting the host cell with a poxvirus of interest under conditions that permit the poxvirus of interest to adsorb to a surface of the host cell;
- and
- culturing the host cell to produce progeny of the poxvirus of interest.
Dependencies are inferred from the claim text.