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US 2021/0279218 A1Published application
DNA Data Storage Using Reusable Nucleic Acids
AssigneeIntegrated Dna Technologies, Inc.
InventorsJoseph Alan WALDER, Jeffrey A. MANTHEY, William E. MARTIN, III, Shawn ALLEN
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Abstract
Disclosed herein are nucleic acid-based data storage systems and nucleic acid data storage constructs comprising reusable nucleic acid sequences, each representing information carried by a single bit (and, in some embodiments, one or more adjacent bits) within a bit string, and each furthermore representing the position of the single bit within the bit string. Also described are methods for storing data in the nucleic acid-based data storage systems and nucleic acid data storage constructs of the disclosure.
Claims22
A method for storing data in a collection of data storage nucleic acids, the method comprising:
- (a) converting the data into a base-B bit string;
- (b) sub-dividing the base-B bit string into y secondary bit strings, and sub-dividing each secondary bit string into x primary bit strings n bits in length;
- (c) for each of x primary bit strings:
- (i) performing one of:
- (A) where B equals 2, such that the base-B bit string is a binary bit string, from a library of n primary data storage nucleic acids, each comprising a single bit-mer sequence, each bit-mer sequence representing a specific position of a primary bit string n bits in length, selecting the primary data storage nucleic acid with the bit-mer sequence for each position of the primary bit string which is a 1, and selecting no primary data storage nucleic acid for each position of the primary bit string which is a 0, wherein each bit-mer sequence is reusable;
- (B) where B equals 2, such that the base-B bit string is a binary bit string, from a library of 2·n primary data storage nucleic acids, each comprising a single bit-mer sequence, each of n bit-mer sequences representing a 1 at a specific position of a primary bit string n bits in length, and each of n other bit-mer sequences representing a 0 at a specific position of the primary bit string, selecting the primary data storage nucleic acid with the 1 bit-mer sequence for each position of the primary bit string which is a 1, and selecting the primary data storage nucleic acid with the 0 bit-mer sequence for each position of the primary bit string which is a 0, wherein each n bit-mer sequence is reusable;
- or
- (C) where B is greater than 2, from a library of B·n primary data storage nucleic acids, each comprising a single bit-mer sequence, each of B·n bit-mer sequences representing a particular base-B digit at a specific position of the primary bit string, selecting the primary data storage nucleic acid with the appropriate bit-mer sequence for each position of the primary bit string, wherein each B·n bit-mer sequence is reusable;
- wherein all primary data storage nucleic acids comprise the same 5′ and 3′ 1° primer binding sequences flanking the bit-mer sequence therein;
- (ii) pooling the selected data storage nucleic acids to form one of x pools of primary data storage nucleic acids storing the data of one of x primary bit strings;
- (iii) using a 1° primer pair that binds to the 5′ and 3′ 1° primer binding sequences to add to each primary data storage nucleic acid the same 5′ and 3′ secondary positional index sequences and, optionally, the same 5′ and 3′ 2° primer binding sequences to produce a pool of secondary data storage nucleic acids,
- wherein each of x pools of secondary data storage nucleic acids comprise different 5′ and 3′ secondary positional index sequences;
- and
- (d) pooling the x pools of secondary data storage nucleic acids corresponding to the x primary bit strings within each of y secondary bit strings into a single pool, to form y pools of secondary data storage nucleic acids, each storing the data of one of y secondary bit strings.
11 more elements
A data storage nucleic acid comprising:
- (a) a reusable bit-mer sequence, which represents information carried by a single bit in a primary bit string, and which further represents a position of the bit within the primary bit string;
- (b) a 5′ secondary positional tag comprising
- (i) a 5′ 1° primer binding sequence flanking the 5′ end of the bit-mer sequence, and
- (ii) a 5′ secondary positional index sequence flanking the 5′ end of the 5′ 1° primer binding sequence;
- and
- (c) a 3′ secondary positional tag comprising
- (i) a 3′ 1° primer binding sequence flanking the 3′ end of the bit-mer sequence, and
- (ii) a 3′ secondary positional index sequence flanking the 3′ end of the 3′ 1° primer binding sequence;
- wherein the 5′ and 3′ secondary positional index sequences represent the position of the primary bit string relative to one or more other primary bit strings within a secondary bit string.
6 more elements
A collection of data storage nucleic acids comprising:
- a plurality of data storage nucleic acids, each data storage nucleic acid comprising one of N different reusable bit-mer sequences,
- wherein each reusable bit-mer sequence represents:
- (a) information carried by a bit in a primary bit string n bits in length and the position p of the bit within the primary bit string, and
- (b) information carried by A adjacent bits in the primary bit string;
- and
- wherein N equals B(A+1)·n where the primary bit string is a base-B bit string, and where each of B(A+1)·n bit-mer sequences represents a base-B digit at a particular position within the primary bit string along with the one or more base-B digits at the positions occupied by the A adjacent bits.
3 more elements
A method for storing data in a collection of data storage nucleic acids, the method comprising:
- (a) converting the data into a base-B bit string;
- (b) sub-dividing the base-B bit string into y secondary bit strings, and sub-dividing each secondary bit string into x primary bit strings n bits in length;
- (c) for each of x primary bit strings:
- (i) from a library of B(A+1)·n primary data storage nucleic acids, each comprising a single reusable bit-mer sequence, each of B(A+1)·n bit-mer sequences representing (1) a particular base-B digit at a specific position p of the primary bit string, in addition to (2) a base-B digit at each of A positions adjacent to position p, selecting the primary data storage nucleic acid with the appropriate bit-mer sequence for each position p of the primary bit string;
- wherein all primary data storage nucleic acids comprise the same 5′ and 3′ 1° primer binding sequences flanking the reusable bit-mer sequence therein;
- (ii) pooling the selected data storage nucleic acids to form one of x pools of primary data storage nucleic acids storing the data of one of x primary bit strings;
- (iii) using a 1° primer pair that binds to the 5′ and 3′ 1° primer binding sequences to add to each primary data storage nucleic acid the same 5′ and 3′ secondary positional index sequences and, optionally, the same 5′ and 3′ 2° primer binding sequences to produce a pool of secondary data storage nucleic acids,
- wherein each of x pools of secondary data storage nucleic acids comprise different 5′ and 3′ secondary positional index sequences;
- and
- (d) pooling the x pools of secondary data storage nucleic acids corresponding to the x primary bit strings within each of y secondary bit strings into a single pool, to form y pools of secondary data storage nucleic acids, each storing the data of one of y secondary bit strings.
7 more elements
Dependencies are inferred from the claim text.