Methods of assaying proteins
AssigneeNautilus Biotechnology, Inc.
InventorParag Mallick
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Abstract
Methods and systems for identifying a protein within a sample are provided herein. A panel of antibodies are acquired, none of which are specific for a single protein or family of proteins. Additionally, the binding properties of the antibodies in the panel are determined. Further, the protein is iteratively exposed to a panel of antibodies. Additionally, a set of antibodies which bind the protein are determined. The identity of the protein is determined using one or more deconvolution methods based on the known binding properties of the antibodies to match the set of antibodies to a sequence of a protein.
Claims26
1-3. (canceled)
A method for identifying a protein of a plurality of proteins, comprising:
- (a) providing a plurality of proteins, wherein a protein of the plurality of proteins is immobilized at a position of an array;
- (b) exposing the protein to a plurality of different affinity reagents, wherein affinity reagents of the plurality of different affinity reagents have a known degree of binding non-specificity for proteins of the plurality of different proteins;
- (c) determining a binding pattern of the affinity reagents bound to the protein;
- and
- (d) identifying the protein using the known degree of binding non-specificity of the affinity reagents and the binding pattern of the affinity reagents bound to the protein.
Dependencies are inferred from the claim text.