Systems and methods for detection of target analytes using selectively cleavable bonds
AssigneeIndiana Biosciences Research Institute
InventorsMichael Joseph Pugia, Zane Baird
Life of this patent
Estimating the term…
Abstract
The invention described herein is directed to methods of isolation and detection of target analytes in a sample. The target analytes are coupled to analyte detection particles which comprise base particles having labels and affinity agents coupled thereto by linker arms. The linker arms form bonds with the labels and target analytes and are cleavable under different label and affinity cleavable conditions. Systems and methods for preparing and using the analyte detection particles are also disclosed.
Claims20
An analyte detection particle for use in performing an analysis of target analytes, comprising:
- a particle;
- a label;
- a label linker arm coupled to the particle and to the label, the label linker arm and the label being joined by a label bond cleavable to separate the label from the particle;
- an affinity agent having an affinity for the target analyte and being functional to couple with the target analyte;
- and
- an affinity linker arm coupled to the particle and to the affinity agent, the affinity linker arm and the affinity agent being joined by an affinity bond;
- the label bond being cleavable under label cleavage conditions which do not cleave the affinity bond and which leave the cleaved labels and the target analytes attached to the particles through the analyte linker arm viable for analysis.
A method for preparation of analyte detection particles comprising:
- reacting a functionalized particle with first and second linker arms having the Structure VI:
- in which R is a non-interfering organic group comprising alkyls, polyamides, polypeptides polyethers and other polymeric chains, and X and Z are optional non-interfering organic groups or hydrogen;
- bonding a label to the first linker arm by formation of an ether bond between the label and the linker arm using the oxygen of the CH2OH group of the first linker arm;
- and
- bonding an affinity agent to the second linker arm by formation of an ether bond between the affinity and the linker arm using the oxygen of the CH2OH group of the second linker arm.
An analyte collection particle for capturing a target analyte, comprising:
- a particle;
- an affinity agent having an affinity for the target analyte and being functional to couple with the target analyte;
- an affinity linker arm coupled to the particle and to the affinity agent, the affinity linker arm and the affinity agent being joined by an affinity bond cleavable under affinity cleavage conditions, the affinity bond being an ether bond;
- a collection particle;
- and
- a collection linker arm coupled to the particle and to the collection particle, the collection linker arm and the collection particle being joined by a collection bond cleavable under collection cleavage conditions which are different from the affinity cleavage conditions, the collection bond being an ether bond.
Dependencies are inferred from the claim text.