Simultaneous detection of protein isoforms and nucleic acids from low starting cell numbers
AssigneeThe Regents of the University of California
InventorsAmy E. Herr, Lin He, Andrew J. Modzelewski, Elisabet Rosàs-Canyelles
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Abstract
Dual nucleic acid and protein isoform measurements are performed on low starting cell numbers (e.g. equivalent to the number of blastomeres composing early embryonic development stages (morula and blastocysts)), comprising integrating fractionation polyacrylamide gel electrophoresis (fPAGE) of 10-100 cells with off-chip analysis of nucleic acids in the nuclei.
Claims20
A polyacrylamide gel-based device that integrates electrophoretic separation of cytoplasmic proteins and extraction of nucleic acids from nuclei, the device comprising a polyacrylamide gel covalently grafted to a polyester film, wherein the gel comprises microwells and corresponding separation lanes, wherein each microwell contains fractionated nuclei of cells, and each corresponding separation lane comprises electrophoretically separated proteins of the cells.
Dependencies are inferred from the claim text.