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US 2021/0278331 A1Published application
Fixable viability dyes and their uses
AssigneeBeckman Coulter, Inc.
InventorsTewfik Miloud, Shankar Pattabhiraman, James Tung
Life of this patent
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Abstract
The present invention provides compositions and methods useful for detecting live cells and dead cells in a cell sample. The methods rely on labelling compounds comprising a maleimide moiety linked to a label.
Claims18
A method for distinguishing dead cells from live cells in a cell sample, the method comprising
- contacting the cell sample with a labelling compound of Formula I:
- wherein R is a label, or a label attached through a linker,
- under conditions whereby the labelling compound binds free thiol groups on cell surface proteins on dead cells and live cells and preferentially crosses cell membranes of dead cells and binds free thiol groups on intracellular proteins in the dead cells;
- and
- detecting the label in the cell sample, thereby distinguishing dead cells from live cells in the cell sample by detecting increased labelling of the dead cells, as compared to live cells.
2 more elements
A kit for distinguishing dead cells from live cells in a cell sample, the kit comprising
- a container comprising a labelling compound of Formula I:
- wherein R is a label, or a label attached through a linker,
- one or more containers comprising reagents for fixing and permeabilizing cells in the sample, and
- instructions on how to distinguish dead cells from live cells in a cell sample using the labelling compound
1 more element
Dependencies are inferred from the claim text.