Rapid amplification methods fornucleic acid of hepatitis b virus

A method for rapid amplification of hepatitis B virus nucleic acid, comprising:

• mixing a sample containing hepatitis B virus with a nucleic acid release agent followed by adding a PCR premix to obtain a reaction solution, the nucleic acid release agent comprising surfactin, potassium chloride, sodium lauryl sulfonate, and ethanol, the PCR premix comprising deoxy-ribonucleoside triphosphate, a forward primer having a sequence as set forth in SEQ ID NO:
• 1, a reverse primer having a sequence as set forth in SEQ ID NO:
• 2, a DNA polymerase, and an amplification buffer;
• placing the reaction solution into a PCR reaction tube to make the reaction solution presented as a thin film with a thickness of less than or equal to 0.1 mm;
• and
• placing the PCR reaction tube in a PCR amplifier for PCR amplification under a reaction condition set as follows:
• initial denaturation at 90-100° C. for 10-600 sec, denaturation at 90-100° C. for 0-1 sec, and annealing and extension at 50-65° C. for 0-1 sec.