CDI Enhanced COVID-19 Test

A method for detecting a novel coronavirus, severe acute respiratory syndrome-Coronavirus-2 (SARS-CoV-2 or COVID-19) in a sample, comprising:

• preparing a master mix for each of a plurality of assays wherein the assays include a first assay that is novel coronavirus (“nCoV”) specific;
• a second assay that is conversed for severe acute respiratory syndrome (“SARS”) related coronaviruses;
• and a third assay that is an internal control;
• loading 15 μL of the master mix to each of a plurality of wells, wherein the wells are defined by a plate map;
• adding 5 μL of ribonucleic acid (“RNA”) to each well as defined by the plate map;
• transferring the sample to a mic qPCR cycler;
• and running the mic cycler.

A method for detecting a novel coronavirus, severe acute respiratory syndrome-Coronavirus-2 (SARS-CoV-2 or COVID-19), comprising:

• providing a CDI Enhanced COVID-19 test that includes:
• using a real-time reverse transcription polymerase chain reaction (RT-PCR) test;
• using one primer and probe set to detect one region in a SARS-CoV-2 nucleocapsid gene (N2);
• using one primer and probe set for universal detection of a SARS-like coronaviruses (E), and
• using one primer and probe set to detect human RNase P (RP) in a clinical sample.