Methods and compositions for identification of tumor models
AssigneeCrown Bioscience (Suzhou) Inc.
InventorsSheng GUO, Xiaobo CHEN, Wubin QIAN, Henry LI
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Abstract
The disclosure provides methods and compositions, e.g., kits, for identifying or authenticating a sample, e.g., a tumor model, based on the genotype of the sample at a group of SNP loci.
Claims18
A method for determining the alleles in a sample, the method comprising:
- obtaining a nucleic acid from the sample;
- selecting a set of single nucleotide polymorphism (SNP) of the sample that can be amplified together in a multiplex amplification reaction, wherein the set of SNP loci are selected from the group as shown in Table 1 or Table 2;
- providing a set of oligonucleotide primer pairs, wherein each oligonucleotide primer pair in the set flanks a single locus in the set of SNP loci, and wherein each oligonucleotide primer pair is capable of amplifying a single locus from the set of SNP loci in a multiplex amplification reaction;
- co-amplifying the set of SNP loci in a multiplex amplification reaction, wherein the product of the multiplex amplification reaction comprises a mixture of amplified alleles from each of the co-amplified loci in the set of SNP loci;
- and
- evaluating the products of the co-amplification reaction to determine the alleles present at each of the loci analyzed in the set of SNP loci within the sample.
A kit for determining the alleles in a sample comprising a set of oligonucleotide primer pairs, wherein each oligonucleotide primer pair in the set flanks a single locus in a set of SNP loci, and wherein each oligonucleotide primer pair is capable of amplifying a single locus from the set of SNP loci in a multiplex amplification reaction, wherein the set of SNP loci are selected from the group as shown in Table 1 or in Table 2;
- and an agent for co-amplifying the set of SNP loci in a multiplex amplification reaction.
Dependencies are inferred from the claim text.