Circulating tumor and tumor stem cell detection using genomic specific probes
AssigneeBoard of Regents, The University of Texas System
InventorRuth KATZ
Life of this patent
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Abstract
The present disclosure comprises a method of detecting circular tumor cells and methods of detecting, evaluating, or staging cancer in a patient, as well as a method of monitoring treatment of cancer in a patient using the claimed method. In other embodiments, the method provides for directed to a method of determining the level of circulating tumor cells (CTCs) in a sample having blood cells from a patient by contacting a sample having blood cells from a patient.
Claims32
A method of detecting circulating tumor cells (CTCs) in a sample containing blood cells comprising:
- (a) selecting CTCs from a sample containing blood cells by assessing nuclear area and/or circularity;
- (b) hybridizing the selected cells with labeled nucleic acid probes for 3p22.1, 10q22.3, chromosome 10 centromeric (cep10) and chromosome 3 centromeric (cep3);
- (c) evaluating the signal pattern for the selected cells by detecting fluorescence in situ hybridization from cells;
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- (d) detecting CTCs based on pattern of hybridization to all four labeled nucleic acid probes to said selected cells.
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A method of determining the level of circulating tumor cells (CTCs) in a sample containing blood cells from a patient by:
- (a) selecting CTCs from a first blood sample by assessing nuclear size and/or circularity and/or DAPI concentration;
- (b) contacting the selected cells with labeled nucleic acid probes for 3p22.1, 10q22.3, chromosome 10 centromeric (cep10) and chromosome 3 centromeric (cep3);
- (c) detecting fluorescence in situ hybridization from cells;
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- (d) quantifying CTCs based on hybridization to all four labeled nucleic acid probes.
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Dependencies are inferred from the claim text.