Methods and compositions for enriching non-host sequences in host samples
AssigneeUniversity of Alaska Fairbanks
InventorsJiguo Chen, Fang Ge
Life of this patent
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Abstract
Disclosed are compositions and methods for enriching a non-host sequence from a host sample. Also disclosed are compositions and methods for detecting a non-host sequence in a host sample. For example, a pathogen can be enriched and detected in a sample taken from a human without knowing what the pathogen is.
Claims21
-74. (canceled)
A method of enriching non-human nucleic acids from a human sample comprising selectively amplifying nucleic acids isolated from the human sample using non-human target primers to form an enriched population of non-human nucleic acids, wherein the non-human target primers do not bind to at least the top 1,000 human B cell transcripts.
A method of enriching non-human nucleic acids from a human sample comprising hybridizing a set of non-human target primers to total RNA or mRNA isolated from the human sample and selectively reverse transcribing total RNA or mRNA isolated from the human sample to form an enriched population of non-human cDNA strands, wherein the set of non-human target primers do not hybridize to at least the top 1,000 human B cell transcripts.
A diagnostic assay, comprising non-human target primers for amplifying nucleic acids from a human sample to form an enriched population of non-human nucleic acids, wherein the non-human target primers do not bind to at least the top 1,000 human B cell transcripts.
Dependencies are inferred from the claim text.