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US 2021/0277469 A1Published application
Sequencing and high resolution imaging
AssigneeApton Biosystems, Inc.
InventorsBryan P. STAKER, Niandong LIU, Manohar R. FURTADO, Rixun FANG, Norman BURNS, Windsor OWENS
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Abstract
Disclosed herein are methods and systems for detection and discrimination of optical signals from a densely packed substrate. There have broad applications for biomolecule detection near or below the diffraction limit of optical systems, including in improving the efficiency and accuracy or polynucleotide sequencing applications.
Claims20
A method for processing or analyzing a plurality of analytes, comprising:
- (a) providing a substrate that supports said plurality of analytes immobilized adjacent thereto at a density of at least 1 molecule per square micrometer
- (b) obtaining at least one optical signal from said substrate over at least one cycle of probes binding to one or more analytes of said plurality of analytes immobilized adjacent to said substrate;
- (c) processing said at least one optical signal to identify said one or more analytes of said plurality of analytes;
- wherein at least 10% of said plurality of analytes are separated from one another by less than λ/(2*N.A.) of said at least one optical signal.
1 more element
A system for processing or analyzing a plurality of analytes, comprising:
- a substrate configured to support said plurality of analytes immobilized adjacent thereto at a density of at least 1 molecule per square micrometer;
- an optical imaging module configured to obtain at least one optical signal from said substrate over at least one cycle of probes binding to one or more analytes of said plurality of analytes immobilized adjacent to said substrate;
- and
- an image processing module configured to process said at least one optical signal to identify said one or more analytes of said plurality of analytes;
- wherein at least 10% of said plurality of analytes are separated from one another by less than λ/(2*N.A.) of said at least one optical signal.
2 more elements
Dependencies are inferred from the claim text.