Kits for detecting bacterial nucleic acid
AssigneeGen-Probe Incorporated
InventorsBarbara Lynn Eaton, Damon Kittredge Getman, Traci Pawlowski
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Abstract
Disclosed are methods for diagnosing Bacterial Vaginosis in a subject comprising performing an assay for the detection of any one or more of Lactobacillus sp., Gardneralla vaginalis, and Eggerthella sp. in a subject sample. Also disclosed are methods and compositions for detecting Lactobacillus sp., Gardneralla vaginalis, and/or Eggerthella nucleic acid in a sample.
Claims20
A kit for the detection of Lactobacillus sp. and G. vaginalis, the kit comprising:
- a plurality of labeled detection probes, wherein the plurality of labeled detection probes comprises
- (a) a first Lactobacillus-specific labeled detection probe that specifically hybridizes to a Lactobacillus sp. target nucleic acid, and
- (b) a first G. vaginalis-specific labeled detection probe that specifically hybridizes to a G. vaginalis target nucleic acid,
- wherein
- the first Lactobacillus-specific labeled detection probe targets a Lactobacillus sp. 16s RNA region corresponding to a region of SEQ ID NO:1 from about nucleotide position 91 to about nucleotide position 265,
- and
- the first G. vaginalis-specific labeled detection probe targets a G. vaginalis 16S rRNA region corresponding to a region of SEQ ID NO:3 from about nucleotide position 964 to about nucleotide position 1036.
A kit for the detection of Lactobacillus sp. and G. vaginalis, the kit comprising:
- a plurality of labeled detection probes, wherein the plurality of labeled detection probes comprises
- (a) a first Lactobacillus-specific labeled detection probe that specifically hybridizes to a Lactobacillus sp. target nucleic acid, wherein the first Lactobacillus-specific labeled detection probe
- (i) comprises a target-hybridizing sequence substantially corresponding to residues 1-21 SEQ ID NO:9,
- (ii) comprises the nucleotide sequence of SEQ ID NO:9, or
- (iii) consists of the nucleotide sequence SEQ ID NO:9;
- and
- (b) a first G. vaginalis-specific labeled detection probe that specifically hybridizes to a G. vaginalis target nucleic acid, wherein the first G. vaginalis-specific labeled detection probe
- (i) comprises a target-hybridizing sequence substantially corresponding to residues 1-19 of SEQ ID NO:14,
- (ii) comprises the nucleotide sequence of SEQ ID NO:14, or
- (iii) consists of the nucleotide sequence of SEQ ID NO:
- 14.
A kit for the detection of Lactobacillus sp., G. vaginalis, and Eggerthella sp., the kit comprising:
- a plurality of labeled detection probes, wherein the plurality of labeled detection probes comprises
- (a) a first Lactobacillus-specific labeled detection probe that specifically hybridizes to a Lactobacillus sp. target nucleic acid, wherein the first Lactobacillus-specific labeled detection probe
- (i) comprises a target-hybridizing sequence substantially corresponding to residues 1-21 SEQ ID NO:9,
- (ii) comprises the nucleotide sequence of SEQ ID NO:9, or
- (iii) consists of nucleotide SEQ ID NO:9;
- (b) a first G. vaginalis-specific labeled detection probe that specifically hybridizes to a G. vaginalis target nucleic acid, wherein the first G. vaginalis-specific labeled detection probe
- (i) comprises a target-hybridizing sequence substantially corresponding to residues 1-19 of SEQ ID NO:14,
- (ii) comprises the nucleotide sequence of SEQ ID NO:14, or
- (iii) consists of the nucleotide sequence of SEQ ID NO:
- 14;
- and
- (c) a first Eggerthella-specific labeled detection probe that specifically hybridizes to an Eggerthella sp. target nucleic acid, wherein the first Eggerthella-specific labeled detection probe
- (i) substantially corresponds to the nucleotide sequence of SEQ ID NO:
- 18,
- (ii) comprises the nucleotide sequence of SEQ ID NO:18, or
- (iii) consists of the nucleotide sequence of SEQ ID NO:18.
Dependencies are inferred from the claim text.