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US 2021/0277448 A1Published application
Compositions, methods, and kits for synthesis and detection of nucleic acids
AssigneeLife Technologies Corporation
InventorsFerrier LE, Kalpith RAMAMOORTHI, Joyce WILDE, Nicole FANTIN, Jordan LANG, David DUPONT, Junko STEVENS
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Abstract
Compositions, methods, and kits for synthesizing, detecting, and/or quantifying nucleic acids are provided herein. Embodiments comprise a nucleic acid amplification composition comprising a thermostable DNA polymerase and agents which improve nucleic acid synthesis, amplification, detection, and/or quantification of nucleic acid targets in a crude extract or crude lysate sample.
Claims27
-59. (canceled)
A composition comprising:
- a thermostable DNA polymerase;
- at least one salt;
- an antifoam agent;
- dGTP;
- and
- deaza-dGTP, wherein the deaza-dGTP is at a concentration between 0.1 and 1.0 mM.
3 more elements
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A method for performing PCR comprising:
- a) contacting the composition of any one of the previous claims with a nucleic acid sample comprising a target polynucleotide and with a target-specific primer to form a reaction mixture;
- and
- b) performing PCR on the reaction mixture to form an amplification product.
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Dependencies are inferred from the claim text.