Engineered microorganisms & methods for improved crotyl alcohol production
AssigneeGenomatica, Inc.
InventorsStephanie J. Culler, Nicholas Eakley, Kevin Gregory Hoff, Robin E. Osterhout, Priti Pharkya, Erik C. Ralph, Stephen J. Van Dien
Life of this patent
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Abstract
Disclosed are methods and engineered microorganisms that enhance or improve the production of crotyl alcohol. The engineered microorganisms include genetic modifications in alcohol dehydrogenase, alkene reductase or both enzymatic activities. By such genetic modifications, a crotyl alcohol production pathway is provided or improved.
Claims21
1-33. (canceled)
A method for producing crotyl alcohol using an engineered microorganism, where crotyl alcohol is a bioproduct, or crotyl alcohol is an intermediate in the bioproduct pathway, the method comprising:
- culturing the engineered microorganism under conditions resulting in crotyl alcohol or use of crotyl alcohol as the intermediate in the bioproduct pathway resulting in bioproduct, wherein the engineered microorganism comprises a genetic modification causing partial or complete loss of activity in an alkene reductase that converts crotonaldehyde to butyraldehyde and at least one exogenous nucleic acid encoding an enzyme of the bioproduct pathway, where the bioproduct pathway is a crotyl alcohol pathway or uses a crotyl alcohol as an intermediate in the bioproduct pathway.
The engineered microorganism of claim 47, wherein the engineered microorganism further comprises at least one genetic modification causing partial or complete loss of activity in (a) an alcohol dehydrogenase (ADH) that converts crotyl alcohol to crotonaldehyde, or (b) an ADH that converts butyraldehyde to butanol, or (c) both combination of (a) and (b).
An engineered microorganism comprising at least one genetic modification causing partial or complete loss of activity in an alkene reductase that converts crotonaldehyde to butyraldehyde and an exogenous nucleic acid encoding an enzyme of a bioproduct pathway, where the bioproduct pathway is a crotyl alcohol pathway or uses a crotyl alcohol as an intermediate in the bioproduct pathway.
Dependencies are inferred from the claim text.