US 2021/0277413 A1 · NUCLEIC ACID MOLECULES THAT CONFER RESISTANCE TO COLEOPTERAN PESTS

Abstract

This disclosure concerns nucleic acid molecules and methods of use thereof for control of coleopteran pests through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in coleopteran pests. The disclosure also concerns methods for making transgenic plants that express nucleic acid molecules useful for the control of coleopteran pests, and the plant cells and plants obtained thereby.

An isolated nucleic acid comprising at least one polynucleotide operably linked to a heterologous promoter, wherein the polynucleotide is selected from the group consisting of:

SEQ ID NO:1;
the complement of SEQ ID NO:1;
a fragment of at least 15 contiguous nucleotides of SEQ ID NO:1;
the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:1;
a native coding sequence of a Coleopteran organism comprising SEQ ID NO:1;
the complement of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:1;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:1;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:1;
native coding sequence of a Coleopteran organism comprising SEQ ID NOs:3-7;
the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:3-7;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:3-7;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:3-7;
SEQ ID NO:8;
the complement of SEQ ID NO:8;
a fragment of at least 15 contiguous nucleotides of SEQ ID NO:8;
the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:8;
a native coding sequence of a Coleopteran organism comprising SEQ ID NO:8;
the complement of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:8;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:8;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:8;
native coding sequence of a Coleopteran organism comprising SEQ ID NOs:10-12;
the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:10-12;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:10-12;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:10-12;
SEQ ID NO:13;
the complement of SEQ ID NO:13;
a fragment of at least 15 contiguous nucleotides of SEQ ID NO:13;
the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:13;
a native coding sequence of a Coleopteran organism comprising SEQ ID NO:13;
the complement of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:13;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:13;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:13;
native coding sequence of a Coleopteran organism comprising SEQ ID NOs:15-16;
the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:15-16;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:15-16;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:15-16;
SEQ ID NO:17;
the complement of SEQ ID NO:17;
a fragment of at least 15 contiguous nucleotides of SEQ ID NO:17;
the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:17;
a native coding sequence of a coleopteran organism comprising SEQ ID NO:17;
the complement of a native coding sequence of a coleopteran organism comprising SEQ ID NO:17;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a coleopteran organism comprising SEQ ID NO:17;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:17;
native coding sequence of a Coleopteran organism comprising SEQ ID NOs:19-20;
the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:19-20;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:19-20;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:19-20;
SEQ ID NO:21;
the complement of SEQ ID NO:21;
a fragment of at least 15 contiguous nucleotides of SEQ ID NO:21;
the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:21;
a native coding sequence of a coleopteran organism comprising SEQ ID NO:21;
the complement of a native coding sequence of a coleopteran organism comprising SEQ ID NO:21;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a coleopteran organism comprising SEQ ID NO:21;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:21;
native coding sequence of a Coleopteran organism comprising SEQ ID NOs:23-25;
the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:23-25;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:23-25;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:23-25;
SEQ ID NO:26;
the complement of SEQ ID NO:26;
a fragment of at least 15 contiguous nucleotides of SEQ ID NO:26;
the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:26;
a native coding sequence of a coleopteran organism comprising SEQ ID NO:26;
the complement of a native coding sequence of a coleopteran organism comprising SEQ ID NO:26;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a coleopteran organism comprising SEQ ID NO:26;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:26;
native coding sequence of a Coleopteran organism comprising SEQ ID NOs:28-29;
the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:28-29;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:28-29;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:28-29;
SEQ ID NO:30;
the complement of SEQ ID NO:30;
a fragment of at least 15 contiguous nucleotides of SEQ ID NO:30;
the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:30;
a native coding sequence of a coleopteran organism comprising SEQ ID NO:30;
the complement of a native coding sequence of a coleopteran organism comprising SEQ ID NO:30;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a coleopteran organism comprising SEQ ID NO:30;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:30;
native coding sequence of a Coleopteran organism comprising SEQ ID NOs:32-34;
the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:32-34;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:32-34;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:32-34;
SEQ ID NO:35;
the complement of SEQ ID NO:35;
a fragment of at least 15 contiguous nucleotides of SEQ ID NO:35;
the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:35;
a native coding sequence of a coleopteran organism comprising SEQ ID NO:35;
the complement of a native coding sequence of a coleopteran organism comprising SEQ ID NO:35;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a coleopteran organism comprising SEQ ID NO:35;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:35;
native coding sequence of a Coleopteran organism comprising SEQ ID NOs:37-39;
the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:37-39;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:37-39;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:37-39;
SEQ ID NO:40;
the complement of SEQ ID NO:40;
a fragment of at least 15 contiguous nucleotides of SEQ ID NO:40;
the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:40;
a native coding sequence of a coleopteran organism comprising SEQ ID NO:40;
the complement of a native coding sequence of a coleopteran organism comprising SEQ ID NO:40;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a coleopteran organism comprising SEQ ID NO:40;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:40;
native coding sequence of a Coleopteran organism comprising SEQ ID NOs:42-44;
the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:42-44;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:42-44;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:42-44;
SEQ ID NO:45;
the complement of SEQ ID NO:45;
a fragment of at least 15 contiguous nucleotides of SEQ ID NO:45;
the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:1;
a native coding sequence of a coleopteran organism comprising SEQ ID NO:45;
the complement of a native coding sequence of a coleopteran organism comprising SEQ ID NO:45;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a coleopteran organism comprising SEQ ID NO:45;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:45;
native coding sequence of a Coleopteran organism comprising SEQ ID NOs:47-49;
the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:47-49;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:47-49;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:47-49;
SEQ ID NO:50;
the complement of SEQ ID NO:50;
a fragment of at least 15 contiguous nucleotides of SEQ ID NO:50;
the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:50;
a native coding sequence of a coleopteran organism comprising SEQ ID NO:50;
the complement of a native coding sequence of a coleopteran organism comprising SEQ ID NO:50;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a coleopteran organism comprising SEQ ID NO:50;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:50;
native coding sequence of a Coleopteran organism comprising SEQ ID NOs:52-53;
the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:52-53;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:52-53;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:52-53;
SEQ ID NO:54;
the complement of SEQ ID NO:54;
a fragment of at least 15 contiguous nucleotides of SEQ ID NO:54;
the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:54;
a native coding sequence of a coleopteran organism comprising SEQ ID NO:54;
the complement of a native coding sequence of a coleopteran organism comprising SEQ ID NO:54;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a coleopteran organism comprising SEQ ID NO:54;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:54;
native coding sequence of a Coleopteran organism comprising SEQ ID NOs:56-58;
the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:56-58;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:56-58;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:56-85;
SEQ ID NO:59;
the complement of SEQ ID NO:59;
a fragment of at least 15 contiguous nucleotides of SEQ ID NO:59;
the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:59;
a native coding sequence of a coleopteran organism comprising SEQ ID NO:59;
the complement of a native coding sequence of a coleopteran organism comprising SEQ ID NO:59;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a coleopteran organism comprising SEQ ID NO:59;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:59;
native coding sequence of a Coleopteran organism comprising SEQ ID NOs:61-63;
the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:61-63;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:61-63;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:61-63;
SEQ ID NO:64;
the complement of SEQ ID NO:64;
a fragment of at least 15 contiguous nucleotides of SEQ ID NO:64;
the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:64;
a native coding sequence of a coleopteran organism comprising SEQ ID NO:64;
the complement of a native coding sequence of a coleopteran organism comprising SEQ ID NO:64;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a coleopteran organism comprising SEQ ID NO:64;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:64;
native coding sequence of a Coleopteran organism comprising SEQ ID NOs:66-67;
the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:66-67;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:66-67;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:66-67;
SEQ ID NO:68;
the complement of SEQ ID NO:68;
a fragment of at least 15 contiguous nucleotides of SEQ ID NO:68;
the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:68;
a native coding sequence of a coleopteran organism comprising SEQ ID NO:68;
the complement of a native coding sequence of a coleopteran organism comprising SEQ ID NO:68;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a coleopteran organism comprising SEQ ID NO:68;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:68;
native coding sequence of a Coleopteran organism comprising SEQ ID NOs:70-71;
the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:70-71;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:70-71;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:70-71;
SEQ ID NO:72;
the complement of SEQ ID NO:72;
a fragment of at least 15 contiguous nucleotides of SEQ ID NO:72;
the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:72;
a native coding sequence of a coleopteran organism comprising SEQ ID NO:72;
the complement of a native coding sequence of a coleopteran organism comprising SEQ ID NO:72;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a coleopteran organism comprising SEQ ID NO:72;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:72;
native coding sequence of a Coleopteran organism comprising SEQ ID NOs:74-75;
the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:74-75;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:74-75;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:74-75;
SEQ ID NO:76;
the complement of SEQ ID NO:76;
a fragment of at least 15 contiguous nucleotides of SEQ ID NO:76;
the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:76;
a native coding sequence of a coleopteran organism comprising SEQ ID NO:76;
the complement of a native coding sequence of a coleopteran organism comprising SEQ ID NO:76;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a coleopteran organism comprising SEQ ID NO:76;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:76;
native coding sequence of a Coleopteran organism comprising SEQ ID NOs:78-79;
the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:78-79;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:78-79;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:78-79;
SEQ ID NO:80;
the complement of SEQ ID NO:80;
a fragment of at least 15 contiguous nucleotides of SEQ ID NO:80;
the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:80;
a native coding sequence of a coleopteran organism comprising SEQ ID NO:80;
the complement of a native coding sequence of a coleopteran organism comprising SEQ ID NO:80;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a coleopteran organism comprising SEQ ID NO:80;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:80;
native coding sequence of a Coleopteran organism comprising SEQ ID NOs:82-85;
the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:82-85;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:82-85;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:82-85;
SEQ ID NO:86;
the complement of SEQ ID NO:86;
a fragment of at least 15 contiguous nucleotides of SEQ ID NO:86;
the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:86;
a native coding sequence of a coleopteran organism comprising SEQ ID NO:86;
the complement of a native coding sequence of a coleopteran organism comprising SEQ ID NO:86;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a coleopteran organism comprising SEQ ID NO:86;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:86;
native coding sequence of a Coleopteran organism comprising SEQ ID NOs:88-89;
the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:88-89;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:88-89;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:88-89;
SEQ ID NO:90;
the complement of SEQ ID NO:90;
a fragment of at least 15 contiguous nucleotides of SEQ ID NO:90;
the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:90;
a native coding sequence of a coleopteran organism comprising SEQ ID NO:90;
the complement of a native coding sequence of a coleopteran organism comprising SEQ ID NO:90;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a coleopteran organism comprising SEQ ID NO:90;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:90;
native coding sequence of a Coleopteran organism comprising SEQ ID NOs:92-93;
the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:92-93;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:92-93;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:92-93;
SEQ ID NO:94;
the complement of SEQ ID NO:94;
a fragment of at least 15 contiguous nucleotides of SEQ ID NO:94;
the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:94;
a native coding sequence of a coleopteran organism comprising SEQ ID NO:94;
the complement of a native coding sequence of a coleopteran organism comprising SEQ ID NO:94;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a coleopteran organism comprising SEQ ID NO:94;
the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NO:94;
native coding sequence of a Coleopteran organism comprising SEQ ID NOs:96-97;
the complement of a native coding sequence of a Coleopteran organism comprising of SEQ ID NOs:96-97;
a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:96-97;
and the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Coleopteran organism comprising SEQ ID NOs:96-97.

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Nucleic acid molecules that confer resistance to coleopteran pests

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Nucleic acid molecules that confer resistance to coleopteran pests

Life of this patent

Abstract

Claims47