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US 2021/0277409 A1Published application
Methods for selecting transformed plants
AssigneePioneer Hi-Bred International, Inc.
InventorsAJITH ANAND, HYEON-JE CHO, THEODORE M KLEIN
Life of this patent
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Abstract
Spectinomycin resistant or streptomycin resistant transgenic plants and methods of making such plants are provided.
Claims52
A plant transformed with a recombinant expression cassette comprising a marker gene cassette comprising a DNA sequence imparting spectinomycin resistance or streptomycin resistance in plants, wherein the DNA sequence comprises a nucleotide sequence selected from the group consisting of:
- (a) at least one of SEQ ID NO:
- 9, SEQ ID NO:
- 11, SEQ ID NO:
- 13, SEQ ID NO:
- 15, SEQ ID NO:
- 21 and SEQ ID NO:
- 23, wherein the nucleotide sequence imparts spectinomycin resistance or streptomycin resistance in plants;
- (b) a nucleotide sequence that is at least 95% identical to at least one of SEQ ID NO:
- 9, SEQ ID NO:
- 11, SEQ ID NO:
- 13, SEQ ID NO:
- 15, SEQ ID NO:
- 21 and SEQ ID NO:
- 23, wherein the nucleotide sequence imparts spectinomycin resistance or streptomycin resistance in plants;
- (c) a nucleotide sequence that is at least 70% identical to at least one of SEQ ID NO:
- 9, SEQ ID NO:
- 11, SEQ ID NO:
- 13, SEQ ID NO:
- 15, SEQ ID NO:
- 21 and SEQ ID NO:
- 23, wherein the nucleotide sequence imparts spectinomycin resistance or streptomycin resistance in plants;
- (d) a nucleotide sequence encoding a polypeptide of at least one of SEQ ID NO:
- 10, SEQ ID NO:
- 12, SEQ ID NO:
- 14, SEQ ID NO:
- 16, SEQ ID NO:
- 22, and SEQ ID NO:
- 24, wherein the nucleotide sequence imparts spectinomycin resistance or streptomycin resistance in plants;
- (e) a nucleotide sequence encoding a polypeptide that is at least 95% identical to at least one of SEQ ID NO:
- 10, SEQ ID NO:
- 12, SEQ ID NO:
- 14, SEQ ID NO:
- 16, SEQ ID NO:
- 22, and SEQ ID NO:
- 24, wherein the nucleotide sequence imparts spectinomycin resistance or streptomycin resistance in plants;
- and
- (f) a nucleotide sequence encoding a polypeptide that is at least 70% identical to at least one of SEQ ID NO:
- 10, SEQ ID NO:
- 12, SEQ ID NO:
- 14, SEQ ID NO:
- 16, SEQ ID NO:
- 22, and SEQ ID NO:
- 24, wherein the nucleotide sequence imparts spectinomycin resistance or streptomycin resistance in plants.
40 more elements
A method of producing a transgenic plant expressing a trait gene cassette comprising:
- transforming a plant cell with a recombinant expression cassette comprising a trait gene cassette and a marker gene cassette, the marker gene cassette comprising a DNA sequence imparting spectinomycin resistance or streptomycin resistance in plants, wherein the DNA sequence comprises a nucleotide sequence selected from the group consisting of:
- (a) at least one of SEQ ID NO:
- 9, SEQ ID NO:
- 11, SEQ ID NO:
- 13, SEQ ID NO:
- 15, SEQ ID NO:
- 21 and SEQ ID NO:
- 23, wherein the nucleotide sequence imparts spectinomycin resistance or streptomycin resistance in plants;
- (b) a nucleotide sequence that is at least 95% identical to at least one of SEQ ID NO:
- 9, SEQ ID NO:
- 11, SEQ ID NO:
- 13, SEQ ID NO:
- 15, SEQ ID NO:
- 21 and SEQ ID NO:
- 23, wherein the nucleotide sequence imparts spectinomycin resistance or streptomycin resistance in plants;
- (c) a nucleotide sequence that is at least 70% identical to at least one of SEQ ID NO:
- 9, SEQ ID NO:
- 11, SEQ ID NO:
- 13, SEQ ID NO:
- 15, SEQ ID NO:
- 21 and SEQ ID NO:
- 23, wherein the nucleotide sequence imparts spectinomycin resistance or streptomycin resistance in plants;
- (d) a nucleotide sequence encoding a polypeptide of at least one of SEQ ID NO:
- 10, SEQ ID NO:
- 12, SEQ ID NO:
- 14, SEQ ID NO:
- 16, SEQ ID NO:
- 22, and SEQ ID NO:
- 24, wherein the nucleotide sequence imparts spectinomycin resistance or streptomycin resistance in plants;
- (e) a nucleotide sequence encoding a polypeptide that is at least 95% identical to at least one of SEQ ID NO:
- 10, SEQ ID NO:
- 12, SEQ ID NO:
- 14, SEQ ID NO:
- 16, SEQ ID NO:
- 22, and SEQ ID NO:
- 24, wherein the nucleotide sequence imparts spectinomycin resistance or streptomycin resistance in plants;
- and
- (f) a nucleotide sequence encoding a polypeptide that is at least 70% identical to at least one of SEQ ID NO:
- 10, SEQ ID NO:
- 12, SEQ ID NO:
- 14, SEQ ID NO:
- 16, SEQ ID NO:
- 22, and SEQ ID NO:
- 24, wherein the nucleotide sequence imparts spectinomycin resistance or streptomycin resistance in plants;
- selecting a spectinomycin resistant or a streptomycin resistant transgenic plant cell;
- and
- regenerating the transgenic plant expressing the trait gene cassette.
44 more elements
The method of 33, wherein the recombinant expression cassette resides in a disarmed Agrobacteria, an Ochrobactrum bacteria or a Rhizobiaceae bacteria.
Dependencies are inferred from the claim text.