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US 2021/0277367 A1Published application
Heat-resistant reverse transcriptase mutant
AssigneeTakara Bio Inc.
InventorsKazuhiko ISHIKAWA, Takashi UEMORI, Nariaki TAKATSU
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AssigneeTakara Bio Inc.
InventorsKazuhiko ISHIKAWA, Takashi UEMORI, Nariaki TAKATSU
Estimating the term…
Provided are: a reverse transcriptase mutant including an amino acid mutation at a position corresponding to position 55 of the amino acid sequence of wild-type reverse transcriptase derived from the Moloney murine leukemia virus, wherein the reverse transcriptase mutant is characterized in that the amino acid mutation is a substitution from threonine to another amino acid, and the other amino acid is selected from the group consisting of amino acids having a nonpolar aliphatic side chain and amino acids having a polar acidic functional group side chain; a nucleic acid that encodes the mutant; a method for producing the mutant and the nucleic acid that encodes the mutant; a method for synthesizing cDNA in which the mutant is used; and a composition and kit including the mutant.
A reverse transcriptase mutant comprising an amino acid mutation at a position corresponding to position 55 of the amino acid sequence of a wild-type Moloney murine leukemia virus (MMLV) reverse transcriptase, wherein the amino acid mutation is a replacement of threonine with a different amino acid and the different amino acid is selected from the group consisting of amino acids having non-polar aliphatic side chains and amino acids having polar acidic functional group side chains.
A method for producing a nucleic acid encoding a reverse transcriptase mutant, the method comprising a step of replacing a codon encoding threonine at a position corresponding to position 55 of the amino acid sequence of a wild-type MMLV reverse transcriptase in a nucleic acid encoding a MMLV reverse transcriptase, with a codon encoding an amino acid selected from the group consisting of amino acids having non-polar aliphatic side chains and amino acids having polar acidic functional group side chains.
A method for producing a heat-resistant reverse transcriptase mutant, the method comprising a step of replacing threonine at a position corresponding to position 55 of the amino acid sequence of a wild-type MMLV reverse transcriptase, with an amino acid selected from the group consisting of amino acids having non-polar aliphatic side chains and amino acids having polar acidic functional group side chains.
A method for increasing heat resistance of a reverse transcriptase, the method comprising a step of replacing a codon encoding threonine at a position corresponding to position 55 of the amino acid sequence of a wild-type MMLV reverse transcriptase in a nucleic acid encoding a MMLV reverse transcriptase, with a codon encoding an amino acid selected from the group consisting of amino acids having non-polar aliphatic side chains and amino acids having polar acidic functional group side chains.
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