Generating virus or other antigen-specific t cells from a naïve t cell population

A process for producing a plurality of tumor-associated antigen-specific T cells comprising:

• obtaining a sample comprising immune cells from a donor;
• separating said immune cells into T-cells and T-cell precursor cells and antigen presenting cells and antigen presenting precursors from said sample;
• reserving the separated T-cells and T-cell precursor cells;
• generating mature dendritic cells comprising contacting the separated antigen presenting cells and antigen presenting precursor cells with IL-4, GM-CSF, LPS and with at least one tumor-associated or tumor-specific antigen to produce a plurality of antigen-presenting dendritic cells that present the at least one tumor-associated or tumor-specific peptide antigen;
• contacting the reserved T-cells and T-cell precursor cells with the plurality of antigen-presenting dendritic cells in the presence of at least IL-7 and IL-15 to produce said plurality of tumor-associated antigen-specific T cells antigen specific T cells that recognize the ate least one peptide antigen;
• and
• recovering said plurality of tumor-associated antigen-specific T cells that recognize the at least one tumor associated or tumor specific peptide antigen.