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US 2021/0277142 A1Published application
Monoclonal antibodies binding mcm5
AssigneeArquer Diagnostics Limited
InventorsRonald Alfred LASKEY, Kai STOEBER
Life of this patent
Estimating the term…
Abstract
The present invention relates to antibodies that bind to Mcm5. The invention further relates to compositions or kits comprising the antibodies, hybridomas capable of expressing the antibodies, polynucleotides, polypeptides and vectors coding for the antibodies and methods for making the antibodies.
Claims21
1-38. (canceled)
An antibody that specifically binds to Minichromosome maintenance 5 protein (Mcm5), wherein the antibody:
- (a) binds to an epitope comprising the amino acid sequence of SEQ ID NO:
- 1 and/or comprises:
- 12A7 CDRH1 which has the amino acid sequence of SEQ ID NO:
- 9,
- 12A7 CDRH2 which has the amino acid sequence of SEQ ID NO:
- 11,
- 12A7 CDRH3 which has the amino acid sequence of SEQ ID NO:
- 13, 12A7 CDRL1 which has the amino acid sequence of SEQ ID NO:
- 3,
- 12A7 CDRL2 which has the amino acid sequence of SEQ ID NO:
- 5, and
- 12A7 CDRL3 which has the amino acid sequence of SEQ ID NO:
- 7;
- or
- (b) binds to an epitope comprising the amino acid sequence of SEQ ID NO:
- 2 and/or comprises:
- 4B4 CDRH1 which has the amino acid sequence of SEQ ID NO:
- 21,
- 4B4 CDRH2 which has the amino acid sequence of SEQ ID NO:
- 23,
- 4B4 CDRH3 which has the amino acid sequence of SEQ ID NO:
- 25,
- 4B4 CDRL1 which has the amino acid sequence of SEQ ID NO:
- 15,
- 4B4 CDRL2 which has the amino acid sequence of SEQ ID NO:
- 17, and
- 4B4 CDRL3 which has the amino acid sequence of SEQ ID NO:
- 19.
25 more elements
A kit comprising:
- (a) (i) a composition which comprises a first antibody that specifically binds to Minichromosome maintenance protein 5 (Mcm5), wherein said first antibody binds to an epitope comprising the amino acid sequence of SEQ ID NO:
- 1 and/or comprises:
- 12A7 CDRH1 which has the amino acid sequence of SEQ ID NO:
- 9,
- 12A7 CDRH2 which has the amino acid sequence of SEQ ID NO:
- 11,
- 12A7 CDRH3 which has the amino acid sequence of SEQ ID NO:
- 13, 12A7 CDRL1 which has the amino acid sequence of SEQ ID NO:
- 3,
- 12A7 CDRL2 which has the amino acid sequence of SEQ ID NO:
- 5, and
- 12A7 CDRL3 which has the amino acid sequence of SEQ ID NO:
- 7;
- and
- (ii) a composition which comprises a second antibody that specifically binds to Mcm5, wherein said second antibody binds to an epitope comprising the amino acid sequence of SEQ ID NO:
- 2 and/or comprises:
- 4B4 CDRH1 which has the amino acid sequence of SEQ ID NO:
- 21,
- 4B4 CDRH2 which has the amino acid sequence of SEQ ID NO:
- 23,
- 4B4 CDRH3 which has the amino acid sequence of SEQ ID NO:
- 25,
- 4B4 CDRL1 which has the amino acid sequence of SEQ ID NO:
- 15,
- 4B4 CDRL2 which has the amino acid sequence of SEQ ID NO:
- 17, and
- 4B4 CDRL3 which has the amino acid sequence of SEQ ID NO:
- 19;
- or
- (b) (i) a composition which comprises a first antibody that specifically binds to Mcm5, wherein said first antibody binds to an epitope comprising the amino acid sequence of SEQ ID NO:
- 1 and/or comprises:
- 12A7 CDRH1 which has the amino acid sequence of SEQ ID NO:
- 9,
- 12A7 CDRH2 which has the amino acid sequence of SEQ ID NO:
- 11,
- 12A7 CDRH3 which has the amino acid sequence of SEQ ID NO:
- 13, 12A7 CDRL1 which has the amino acid sequence of SEQ ID NO:
- 3,
- 12A7 CDRL2 which has the amino acid sequence of SEQ ID NO:
- 5, and
- 12A7 CDRL3 which has the amino acid sequence of SEQ ID NO:
- 7;
- or
- a composition which comprises a first antibody that specifically binds to Mcm5, wherein said first antibody binds to an epitope comprising the amino acid sequence of SEQ ID NO:
- 2 and/or comprises:
- 4B4 CDRH1 which has the amino acid sequence of SEQ ID NO:
- 21,
- 4B4 CDRH2 which has the amino acid sequence of SEQ ID NO:
- 23,
- 4B4 CDRH3 which has the amino acid sequence of SEQ ID NO:
- 25,
- 4B4 CDRL1 which has the amino acid sequence of SEQ ID NO:
- 15,
- 4B4 CDRL2 which has the amino acid sequence of SEQ ID NO:
- 17, and
- 4B4 CDRL3 which has the amino acid sequence of SEQ ID NO:
- 19;
- and
- (ii) a composition which comprises a second antibody which specifically binds to Mcm5.
56 more elements
A method of using a first antibody and a second antibody to determine the concentration or amount of Minichromosome maintenance 5 protein (Mcm5) in a sample, wherein said method comprises the following steps:
- (i) providing a first antibody immobilized to a solid support, which is an antibody that specifically binds to Mcm5 and which binds to an epitope having the amino acid sequence of SEQ ID NO:
- 2 or SEQ ID NO:
- 1;
- (ii) exposing the sample to the immobilized first antibody such that Mcm5 in the sample may bind to the immobilized antibody to provide immobilized Mcm5;
- (iii) providing a second antibody conjugated to a label, which is an antibody that specifically binds to Mcm5 and which binds to an epitope having an amino acid sequence of SEQ ID NO:
- 2 or SEQ ID NO:
- 1;
- (iv) exposing the immobilized Mcm5 to the labelled second antibody such that the labelled second antibody may bind to the Mcm5;
- and
- (v) detecting the concentration of the labelled second antibody.
7 more elements
Dependencies are inferred from the claim text.