Ulinastatin polypeptides
AssigneeDiaMedica USA Inc.
InventorsRick PAULS, Todd VERDOORN, Karl Frank JOHNSON, Gregory Thomas BLECK
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Abstract
Provided are ulinastatin glycoforms, ulinastatin fusion polypeptides, and related compositions, mixtures, and methods of use, including methods of recombinantly producing ulinastatin polypeptides and treating diseases.
Claims46
An isolated, mature ulinastatin polypeptide, comprising (i) a modified O-linked glycosylation site at residues Glu-Gly-Ser-Gly (SEQ ID NO:
- 10) which reduces glycosylation at the O-linked glycosylation site, (ii) an N-linked glycan at residue at residue N45, and (iii) an O-linked glycan at residue T17, the residues being defined by SEQ ID NO:
- 2 or 4, wherein the ulinastatin polypeptide has at least one ulinastatin activity.
A ulinastatin fusion polypeptide, comprising, in an N-terminal to C-terminal orientation, a bovine alpha-lactalbumin signal peptide and a ulinastatin polypeptide, optionally wherein the ulinastatin polypeptide comprises a modified O-linked glycosylation site at residues Glu-Gly-Ser-Gly (SEQ ID NO:
- 10) which reduces glycosylation at the O-linked glycosylation site, wherein the ulinastatin polypeptide has at least one ulinastatin activity.
(canceled)
Dependencies are inferred from the claim text.