Methods and compositions for delivery of agents across the blood-brain barrier
AssigneeThe Brigham and Women S Hospital, Inc.
InventorFengfeng Bei
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Abstract
The present invention is based on the development of artificial targeting sequences that enhance permeation of agents into cells and across the blood brain barrier, compositions comprising the sequences, and methods of use thereof. Provided herein is an AAV comprising a capsid protein comprising a targeting sequence and a transgene, preferably a therapeutic or diagnostic transgene. Further, provided herein are methods of delivering a transgene to a cell, the method comprising contacting the cell with an AAV or fusion protein described herein.
Claims30
An AAV capsid protein comprising an amino acid sequence that comprises at least four contiguous amino acids from the sequence TVSALFK (SEQ ID NO:8);
- TVSALK (SEQ ID NO:4);
- KLASVT (SEQ ID NO:83);
- or KFLASVT (SEQ ID NO:84).
A targeting sequence comprising V[S/p][A/m/t/]L (SEQ ID NO:79), TV[S/p][A/m/t/]L (SEQ ID NO:80), TV[S/p][A/m/t/]LK (SEQ ID NO:81), or TV[S/p][A/m/t/]LFK. (SEQ ID NO:82).
Dependencies are inferred from the claim text.