Device and method for detecting nucleic acids by isothermal amplification technique
AssigneeTDK Corporation
InventorsFei ZHOU, Xianwen FENG, Zhongjun WAN, Yu FU
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Abstract
A device and a method for detecting nucleic acids by an isothermal amplification technique including a sample processor and a magneto-sensitive detector, wherein the sample processor includes a microfluidic tank, a temperature controller, a capture chip storage chamber, a DNA-modified magnetic bead storage chamber and a cleaning solution storage chamber. The detecting nucleic acids using the device have a fast reaction speed and stable signal output.
Claims20
A device for detecting nucleic acids, wherein the device comprises a sample processor and a magneto-sensitive detector, the sample processor comprises a microfluidic tank, a temperature controller, a capture chip storage chamber, a DNA-modified magnetic bead storage chamber and a cleaning solution storage chamber;
- the microfluidic tank is provided with a reagent inlet and a reagent outlet;
- the temperature controller is disposed on the microfluidic tank;
- an inlet of the capture chip storage chamber is in communication with the reagent outlet of the microfluidic tank through a first micro-channel, is in communication with the DNA-modified magnetic bead storage chamber through a second micro-channel, and is in communication with the cleaning solution storage chamber through a third micro-channel;
- the first micro-channel, the second micro-channel and the third micro-channel are provided with valves to control a reagent in the microfluidic tank, a DNA-modified magnetic bead and a cleaning solution to flow into the capture chip storage chamber, respectively;
- and
- the magneto-sensitive detector comprises a magnetic sensor and a groove for accommodating the capture chip storage chamber, the capture chip storage chamber is inserted inside the groove, and the magnetic sensor senses the DNA-modified magnetic bead in the capture chip storage chamber and converts a magnetic signal of the DNA-modified magnetic bead into an electrical signal.
A device for detecting nucleic acids, wherein the device comprises a sample processor and a magneto-sensitive detector, the sample processor comprises a microfluidic tank, a temperature controller, a capture chip storage chamber, a DNA-modified magnetic bead storage chamber and a cleaning solution storage chamber;
- the microfluidic tank is provided with a reagent inlet and a reagent outlet;
- the temperature controller is disposed on the microfluidic tank;
- an inlet of the capture chip storage chamber is in communication with the reagent outlet of the microfluidic tank through a first micro-channel, is in communication with the DNA-modified magnetic bead storage chamber through a second micro-channel, and is in communication with the cleaning solution storage chamber through a third micro-channel;
- the first micro-channel, the second micro-channel and the third micro-channel are provided with valves to control a reagent in the microfluidic tank, a DNA-modified magnetic bead and a cleaning solution to flow into the capture chip storage chamber, respectively;
- and
- the magneto-sensitive detector comprises a magnetic sensor, and the magnetic sensor senses the DNA-modified magnetic bead in the capture chip storage chamber and converts a magnetic signal of the DNA-modified magnetic bead into an electrical signal.
A method for detecting nucleic acids, wherein the method comprises the following steps:
- (1) mixing a DNA to be tested with a PCR reaction solution in a microfluidic tank to obtain a mixed solution;
- (2) subjecting the mixed solution obtained in step (1) to an isothermal amplification reaction in the microfluidic tank, and after the reaction is completed, adding a nuclease into the microfluidic tank, and cleaving a product obtained by the isothermal amplification reaction into a DNA fragment of a predetermined length;
- (3) reacting the DNA fragment of a predetermined length obtained in step (2) with a capture chip containing a capture DNA to obtain a capture chip containing the DNA fragment of a predetermined length;
- (4) after the reaction is completed, washing away an unbound DNA fragment with a cleaning solution;
- (5) reacting the capture chip containing the DNA fragment of a predetermined length obtained in step (3) with a DNA-modified magnetic bead;
- and
- (6) detecting a signal by a magneto-sensitive detector.
Dependencies are inferred from the claim text.