Optical positioning code disk, device and method for microfluidic chip

An optical positioning device for a microfluidic chip, comprising:

• a code disk, a positioning pin, a rotating shaft, a motor, an internal photoelectric switch and an external photoelectric switch;
• wherein a positioning surface is provided on the rotating shaft;
• wherein a cross section of an outer contour of the code disk is circular, and N light transmissive openings are arranged uniformly around the code disk, wherein a length of a first light transmissive opening is greater than a length of each of a second light transmissive opening to an N-th light transmissive opening, the first light transmissive opening corresponds to a first reaction tank of the microfluidic chip, and the second light transmissive opening to the N-th light transmissive opening correspond to a second reaction tank to an N-th reaction tank of the microfluidic chip respectively;
• wherein
• the motor is fixedly connected with an end of the rotating shaft, and the other end of the rotating shaft passes through a center of the cross section of the code disk;
• positioning is performed by providing the positioning pin at a positioning gap of the microfluidic chip to cooperate with the positioning surface;
• the internal photoelectric switch and the external photoelectric switch are co d to identify the light transmissive openings on the code disk.

An optical positioning method for performing an optical positioning on a microfluidic chip by using an optical positioning device,

• wherein the optical positioning device comprises:
• a code disk, a positioning pin, a rotating shaft, a motor, an internal photoelectric switch and an external photoelectric switch;
• wherein a positioning surface is provided on the rotating shaft;
• wherein a cross section of an outer contour of the code disk is circular, and N light transmissive openings are arranged uniformly around the code disk, wherein a length of a first light transmissive opening is greater than a length of each of a second light transmissive opening to an Nth light transmissive opening, the first light transmissive opening corresponds to a first reaction tank of the microfluidic chip, and the second light transmissive opening to the N-th light transmissive opening correspond to a second reaction tank to an N-th reaction tank of the microfluidic chip respectively;
• wherein
• the motor is fixedly connected with an end of the rotating shaft, and the other end of the rotating shaft passes through a center of the cross section of the code disk;
• positioning is performed by providing the positioning pin at a positioning gap of the microfluidic chip to cooperate with the positioning surface;
• the internal photoelectric switch and the external photoelectric switch are configured to identify the light transmissive openings on the code disk;
• wherein the optical positioning method comprising:
• step 1, making the light transmissive openings of the code disk be corresponding to the reaction tanks of the microfluidic chip respectively based on an angle relationship between the positioning surface on the rotating shaft and the positioning pin;
• step 2, mounting the internal photoelectric switch and the external photoelectric switch, to make the internal photoelectric switch be able to identify the first light transmissive opening corresponding to the first reaction tank, and to make the external photoelectric switch be able to identify the second light transmissive opening to the N-th light transmissive opening corresponding to the second reaction tank to the N-th reaction tank respectively;
• and
• step 3, starting the motor, to make the code disk and the microfluidic chip rotate synchronously, and performing data collection.