Mitochondrial augmentation therapy of ocular diseases
AssigneeMinovia Therapeutics Ltd.
InventorsNatalie Yivgi Ohana, Uriel Halavee, Shmuel Bukshpan, Noa Sher, Moriya Blumkin
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Abstract
The present invention provides stem cells enriched with healthy functional mitochondria, pharmaceutical compositions comprising these cells and methods of use thereof for treating ocular diseases, disorders and symptoms thereof where the disease may or may not be associated with acquired mitochondrial dysfunction.
Claims30
-3. (canceled)
The method of claim 32, wherein the human exogenous mitochondria are syngeneic or allogeneic.
-6. (canceled)
The method of claim 32, wherein the disease or disorder is selected from the group consisting of Chronic Progressive External Ophthalmoplegia (CPEO), dominant optic atrophy (DOA), Kjer optic neuropathy, Retinitis Pigmentosa, age-related macular degeneration (AMD), diabetic retinopathy, cataracts, glaucoma and Pigmentary Retinopathy.
(canceled)
The method of claim 32, wherein the symptom is selected from the group consisting of ptosis, tunnel vision, reduced eye motility, severe external ophthalmoplegia, strabismus, nystagmus, low visual acuity, refractive error, photophobia, partial or total optic atrophy, night blindness, cataracts, blindness, central vision loss, light sensitivity, death of retinal ganglion cells or retinal epithelial cells, optic atrophy, optic neuropathy, retinopathy and blurring and clouding of vision.
(canceled)
The method of claim 32, wherein said pharmaceutical composition is administered directly to the eye, to the ocular system or by systemic administration.
-15. (canceled)
The method of claim 32, wherein the mitochondrially-enriched human stem cells have at least one of:
- (i) an increased mitochondrial DNA content;
- (ii) an increased level of citrate synthase (CS) activity;
- (iii) an increased content of at least one mitochondrial protein selected from SDHA and COX1;
- (iv) an increased rate of O2 consumption;
- (v) an increased rate of ATP production;
- or
- (vi) any combination thereof,
- relative to the corresponding level in the stem cells prior to mitochondrial enrichment.
-19. (canceled)
The method of claim 32, wherein the human stem cells are hematopoietic stem cells, mesenchymal stem cells, pluripotent stem cells (PSCs), induced pluripotent stem cells (iPSCs), or CD34+ cells.
-24. (canceled)
The method of claim 32, wherein the human stem cells are isolated, derived or obtained from cells of the bone marrow, adipose tissue, oral mucosa, skin fibroblasts, blood or umbilical cord blood.
The method of claim 32, wherein the human exogenous mitochondria are isolated or obtained from placenta, placental cells grown in culture or blood cells.
(canceled)
The method of claim 32, wherein the human exogenous mitochondria constitute at least 1-30% of the total mitochondria in the mitochondrially-enriched human stem cells.
The method of claim 32, wherein the composition further comprises non-enriched stem cells, megakaryocytes, erythrocytes, mast cells, myeloblasts, basophils, neutrophils, eosinophils, monocytes, macrophages, natural killer (NK) cells, small lymphocytes, T lymphocytes, B lymphocytes, plasma cells, reticular cells, or any combination thereof.
(canceled)
A method for treating an ocular disease or an ocular disorder or a symptom thereof in a patient in need thereof, comprising administering a pharmaceutical composition to the patient, the pharmaceutical composition comprising at least about 104 to 108 human stem cells, wherein the human stem cells are enriched with human exogenous mitochondria, and wherein the ocular disease or disorder is not a mitochondrial disease or disorder caused by a pathogenic mutation in mitochondrial DNA or by a pathogenic mutation in nuclear DNA encoding a mitochondrial protein.
-35. (canceled)
Dependencies are inferred from the claim text.