Biomolecule Coated Particles and Films and Uses Thereof

A polymeric particle comprising:

• a polymeric core;
• a nucleic acid-polymer conjugate comprising a first single stranded nucleic acid covalently attached to a polymer, wherein the polymer is non-covalently associated with the polymeric core thereby presenting the first single stranded nucleic acid on a surface of the polymeric core;
• and
• a first binding member-nucleic acid conjugate comprising a second single stranded nucleic acid covalently attached to the first binding member, wherein the second single stranded nucleic acid is complementary to the first single stranded nucleic acid and is associated with the first single stranded nucleic acid via hybridization thereby presenting the first binding member on a surface of the polymeric particle,
• wherein the first binding member is a member of a specific-binding pair, wherein the first binding member specifically binds to a second binding member that is a member of the specific-binding pair.

A method of making a polymeric particle, the method comprising:

• covalently linking a nucleic acid to a first polymer to generate a nucleic acid-polymer conjugate, wherein the nucleic acid is a first single stranded nucleic acid;
• sonicating a solution comprising the nucleic acid-polymer conjugate and a second polymer to generate polymeric particles comprising a polymeric core comprising the second polymer, wherein the polymer region of the nucleic acid-polymer conjugate is non-covalently associated with the polymeric core thereby presenting the first single stranded nucleic acid on a surface of the polymeric core;
• attaching to the polymeric core a second single stranded nucleic acid having a sequence complementary to the first single stranded nucleic acid by hybridization;
• and
• covalently or non-covalently attaching the second single stranded nucleic acid to a first binding member of a specific-binding pair to generate the polymeric particle.

A method of making a polymeric particle comprising peptide, polypeptide, and/or nucleic acid encapsulated in a polymeric core and a nucleic acid-polymer conjugate comprising a first single stranded nucleic acid covalently attached to a first polymer, wherein the polymer is non-covalently associated with the polymeric core thereby presenting the first single stranded nucleic acid on a surface of the polymeric core, the method comprising:

• sonicating a solution comprising the peptide, polypeptide, and/or nucleic acid and a second polymer;
• adding the nucleic acid-polymer conjugate to the solution and further sonicating the solution to generate polymeric particles comprising a polymeric core comprising the second polymer and encapsulating the peptide, polypeptide, and/or nucleic acid,
• wherein the polymer region of the nucleic acid-polymer conjugate is non-covalently associated with the polymeric core thereby presenting the first single stranded nucleic acid on a surface of the polymeric core;
• attaching to the polymeric core a second single stranded nucleic acid having a sequence complementary to the first single stranded nucleic acid by hybridization;
• and
• covalently or non-covalently attaching the second single stranded nucleic acid to a first binding member of a specific-binding pair to generate the polymeric particle.

A CAR-T cell for use in a method of treatment of a subject, wherein the method comprises:

• contacting a CAR-antigen presenting particle with a population of T cells comprising the CAR-T cell ex vivo;
• and
• administering the CAR-T cell to the subject following proliferation,
• wherein the CAR-antigen presenting particle comprises a first binding member presented on a surface of a synthetic particle,
• wherein the first binding member is an antigen that specifically binds to a CAR expressed on the CAR-T cell.

A biomolecule-coated film comprising:

• a polymeric film comprising one or more pores;
• a nucleic acid-polymer conjugate comprising a first single stranded nucleic acid covalently attached to a polymer, wherein the polymer is non-covalently associated with the polymeric film thereby presenting the first single stranded nucleic acid on a surface of the polymeric film;
• and
• a first biomolecule-nucleic acid conjugate comprising a second single stranded nucleic acid covalently attached to a biomolecule, wherein the second single stranded nucleic acid is complementary to the first single stranded nucleic acid and is associated with the first single stranded nucleic acid via hybridization thereby presenting the first biomolecule on a surface of the polymeric film.

A method of making a biomolecule coated film, the method comprising:

• covalently linking a nucleic acid to a first polymer to generate a nucleic acid-polymer conjugate, wherein the nucleic acid is a first single stranded nucleic acid;
• mixing a solution comprising the nucleic acid-polymer conjugate and a second polymer in a solvent;
• film casting the solution to generate a polymeric film comprising the second polymer, wherein the polymer region of the nucleic acid-polymer conjugate is non-covalently associated with the polymeric film thereby presenting the first single stranded nucleic acid on a surface of the polymeric film;
• attaching to the polymeric film a second single stranded nucleic acid having a sequence complementary to the first single stranded nucleic acid by hybridization;
• and
• covalently or non-covalently attaching the second single stranded nucleic acid to a biomolecule to generate the biomolecule coated film.