Method of decellularization of kidney tissues, decellularized material by the method and bioink comprising the decellularized material
AssigneePostech Research and Business Development Foundation
InventorsDong-Woo CHO, Jae Yun KIM, Jae Yeon LEE
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Abstract
The method for decellularization of kidney tissue according to the present invention, the decellularized material produced through the method, and a bioink comprising the decellularized material have the effect of maximizing the effect of kidney treatment by maximizing the content of components specialized for kidney treatment such as the collecting duct and renal tubule of the kidney.
Claims17
A method for decellularization of kidney tissue, comprising:
- a step of preparing kidney tissue, in which the kidney tissue extracted from a mammal to be a subject of decellularization is cut into small pieces;
- a step of removing cells, in which the cells of the kidney tissue are converted to a decellularized material using a decellularization solution that comprises a surfactant and a hypertonic solution;
- a step of sterilization, in which the decellularized material and a sterilization solution are stirred such that the microorganisms present in the decellularized material are killed;
- and
- a step of pH adjustment and dilution, in which an enzymatic reaction is performed such that the glycoproteins in the decellularized material are decomposed.
A decellularized material of kidney tissue produced by performing:
- a step of preparing kidney tissue, in which the kidney tissue extracted from a mammal to be a subject of decellularization is cut into small pieces;
- a step of removing cells, in which the cells of the kidney tissue are converted to a decellularized material using a decellularization solution that comprises a surfactant and a hypertonic solution;
- a step of sterilization, in which the decellularized material and a sterilization solution are stirred such that the microorganisms present in the decellularized material are killed;
- and
- a step of pH adjustment and dilution, in which an enzymatic reaction is performed such that the glycoproteins in the decellularized material are decomposed.
A bioink comprising a decellularized material of kidney tissue, wherein the bioink comprises a hydrogel configured to be 3D printed with the decellularized material, wherein the decellularized material is produced by performing:
- a step of preparing kidney tissue, in which the kidney tissue extracted from a mammal to be a subject of decellularization is cut into small pieces;
- a step of removing cells, in which the cells of the kidney tissue are converted to a decellularized material using a decellularization solution that comprises a surfactant and a hypertonic solution;
- a step of sterilization, in which the decellularized material and a sterilization solution are stirred such that the microorganisms present in the decellularized material are killed;
- and
- a step of pH adjustment and dilution, in which an enzymatic reaction is performed such that the glycoproteins in the decellularized material are decomposed.
Dependencies are inferred from the claim text.