Sideroflexins as mitochondrial serine transporters
AssigneeWhitehead Institute for Biomedical Research
InventorsDavid M. Sabatini, Nora Kory
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Abstract
In some aspects, the disclosure provides methods for modulating mitochondrial transport of serine in a cell, the methods comprising modulating expression or activity of one or more sideroflexins. In some aspects, methods of identifying agents that modulate sideroflexin expression or activity are provided. In some aspects, methods of treating cancer are provided.
Claims84
A method for modulating mitochondrial transport of serine in a cell, the method comprising modulating expression or activity of one or more sideroflexins, thereby modulating the mitochondrial transport of serine in the cell.
A method of treating a subject in need of treatment for a cancer, the method comprising modulating expression or activity of one or more sideroflexins in a cancer cell of the subject.
A method of screening one or more test agents to identify a modulator of SFXN1, comprising the steps of:
- a. contacting a test agent with SFXN1,
- b. measuring the level or activity of the contacted SFXN1, and
- c. identifying the test agent as a modulator of SFXN1 if the level or activity of the contacted SFXN1 is decreased or increased as compared to SFXN1 not contacted with the test agent.
A method of screening one or more test agents to identify a candidate anti-cancer agent, comprising contacting the test agent with a cell comprising SFXN1, measuring the survival or proliferation of the contacted cell, and identifying the test agent as a candidate anti-cancer agent if the survival or proliferation of the contacted cell is decreased as compared to the survival or proliferation of a control cell not comprising SFXN1 contacted with the test agent.
A method for modulating mitochondrial transport of cysteine in a cell, the method comprising modulating expression or activity of one or more sideroflexins, thereby modulating the mitochondrial transport of cysteine in the cell.
The method of claim 651, wherein the one or more sideroflexins is selected from the group consisting of SFXN1, SFXN2, SFXN3, SFXN4, and SFXN5.
Dependencies are inferred from the claim text.