Dna preservation in biological specimens using metal chelators
AssigneeNortheastern University Center for Research Innovation, Northeastern Univ.
InventorDaniel Distel
Life of this patent
Estimating the term…
Abstract
Disclosed is a method for preserving high molecular weight DNA in biological tissue, comprising contacting for a period of time at a temperature the biological tissue with an aqueous solution comprising a compound having the structure:
Claims29
A method for preserving high molecular weight DNA in biological tissue, comprising contacting for a period of time at a temperature the biological tissue with an aqueous solution comprising a compound having the structure:
- wherein
- X is alkyl, alkyl-X1-alkyl, alkyl-X1-alkyl-X1-alkyl, aryl-X1-alkyl-X1-aryl, or X2-alkyl-X2, provided that when X is X2-alkyl-X2, then each Y is CH;
- X1, for each occurrence, is independently O, C(H)(R3), or NR4;
- X2, for each occurrence, is N;
- Y, for each occurrence, is independently CH or N;
- R1 is —CO2H, -alkyl-OH, or -alkyl-CO2H, provided that when X is X2-alkyl-X2, then when
- R1 is —CO2H, then the adjacent Y is CH;
- R2 is —CO2H, -alkyl-OH, or -alkyl-CO2H, provided that when X is X2-alkyl-X2, then when
- R2 is —CO2H, then the adjacent Y is CH;
- R3 is —OH, —CO2H, or -alkyl-CO2H;
- and
- R4 is -alkyl-CO2H.
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(canceled)
A method for preserving high molecular weight deoxyribonucleic acid (DNA), comprising contacting for a period of time at a temperature the high molecular weight DNA with an aqueous solution comprising a compound having the structure:
- wherein
- X is alkyl, alkyl-X1-alkyl, alkyl-X1-alkyl-X1-alkyl, aryl-X1-alkyl-X1-aryl, or X2-alkyl-X2, provided that when X is X2-alkyl-X2, then each Y is CH;
- X1, for each occurrence, is independently O, C(H)(R3), or NR4;
- X2, for each occurrence, is N;
- Y, for each occurrence, is independently CH or N;
- R1 is —CO2H, -alkyl-OH, or -alkyl-CO2H, provided that when X is X2-alkyl-X2, then when
- R1 is —CO2H, then the adjacent Y is CH;
- R2 is —CO2H, -alkyl-OH, or -alkyl-CO2H, provided that when X is X2-alkyl-X2, then when
- R2 is —CO2H, then the adjacent Y is CH;
- R3 is —OH, —CO2H, or -alkyl-CO2H;
- and
- R4 is -alkyl-CO2H.
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A method for preserving high molecular weight DNA in biological tissue, comprising contacting for a period of time at a temperature the biological tissue with an aqueous solution comprising a compound having the structure:
- wherein
- n is 0 or 1;
- m is 0 or 1;
- Z is present or absent and when present is N or CR6;
- R5 is alkyl, —CO2H, -alkyl-OH, or -alkyl-CO2H, provided that when R5 is-CO2H, then Z is CR6;
- and
- R6 is —H or —OH, or for preserving high molecular weight deoxyribonucleic acid (DNA), comprising contacting for a period of time at a temperature the high molecular weight DNA with an aqueous solution comprising a compound having the structure:
- wherein
- n is 0 or 1;
- m is 0 or 1;
- Z is present or absent and when present is N or CR6;
- R5 is alkyl, —CO2H, -alkyl-OH, or -alkyl-CO2H, provided that when R5 is-CO2H, then Z is CR6;
- and
- R6 is —H or —OH.
-66. (canceled)
Dependencies are inferred from the claim text.