Assay
AssigneeLumiraDx UK Ltd.
InventorsPhill Lowe, Steven Alexander Keatch, Brian McGuigan, Lois Bello, Chris Slevin, David Lang, John Dilieen
Life of this patent
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Abstract
The present invention relates to methods for detecting an analyte present in a fluid sample using a microfluidic device comprising a detection zone characterized by an optically transmissible portion and reagent(s) associated with a porous matrix, wherein the analyte is detected with an optical detector. The present invention also provides a microfluidic channel and a microfluidic cartridge for use in such a method.
Claims25
-24. (canceled)
A kinetic assay method for use in detecting an analyte within a sample, the method comprising:
- a) providing a sample to a detection zone of a microfluidic channel, the detection zone comprising an optically transmissible portion and a porous matrix comprising one or more reagent(s) localised to an inner luminal surface of the optically transmissible portion of the microfluidic channel, wherein the reagent(s) is/are capable of reacting with the analyte or a reaction product thereof to form a reagent reaction product, the reagent reaction product capable of being detected, using an optical detector which is extra luminal to the optically transmissible portion of the microfluidic channel;
- b) taking at least one optical measurement of the reagent reaction product through the optically transmissible portion;
- and
- detecting any analyte, based upon the at least one optical measurement of the reagent reaction product.
An assay method for use with a sample, the method comprising;
- inserting a cartridge into a reader device, the cartridge comprising a microfluidic channel comprising:
- a detection zone for receiving at least a portion of a sample provided to the microfluidic channel, the detection zone comprising a optically transmissible portion and a porous matrix comprising one or more reagent(s) localized to an inner luminal surface of the optically transmissible portion, wherein the reagent(s) is/are is/are capable of reacting with the analyte or an analyte reaction product thereof to form a reagent reaction product;
- the reagent reaction product capable of being optically detected,
- optionally expelling air from the microfluidic channel using force application means within the reader device;
- introducing the sample to a first end of the microfluidic channel;
- drawing the sample along the microfluidic channel to the detection zone using means within the reader device;
- permitting said analyte or analyte reaction product in the sample to react with the reagent(s) to form the reagent reaction product;
- taking at least one optical measurement of the reagent reaction product using an optical detection device within the reader, the optical detection device being extra luminal to the optically transmissible portion;
- and
- detecting any analyte or analyte reaction product based upon the at least one optical measurement of the reagent reaction product.
Dependencies are inferred from the claim text.