Cartridge and analyzer for fluid analysis

A biosensor analyzer, comprising:

• first and second syringes coupled to first and second fluidic channels of a fluidic cartridge;
• a first needle aligned with the first syringe and a first fluid inlet of the first fluidic channel;
• a second needle aligned with the second syringe and a second fluid inlet of the second fluidic channel;
• first and second actuators configured to control the operation of the first and second syringes, respectively;
• a sensing module coupled to a biological field effect transistor (BioFET) sensor array of the fluidic cartridge, wherein the sensing module is configured to activate the BioFET sensor array;
• and
• a processor electrically coupled to the sensing module, wherein the processor is configured to determine a concentration level of a given analyte from a sample in the fluidic cartridge based on the signals received from the BioFET sensor array.

A method of detecting an analyte in a sample, comprising:

• introducing a first solution through a first inlet into a first fluidic channel to direct the first solution to a biological field effect transistor (BioFET) sensor array;
• measuring a first signal from the BioFET sensor array in the first solution;
• introducing a sample to the first fluidic channel via a sample inlet coupled to the first fluidic channel;
• introducing a second solution through a second inlet into a second fluidic channel to mix with the sample and flow to the BioFET sensor array through a third fluidic channel;
• introducing a third solution through the first inlet into the first fluidic channel to wash the sample away from the BioFET sensor array;
• measuring a second signal from the BioFET sensor array in the third solution;
• and
• comparing the first and second signals to determine the presence of an analyte in the sample.