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US 2021/0263013 A1Published application
Use of eomesodermin to determine risk of allograft rejection
AssigneeUniversity of Pittsburgh—Of the Commonwealth System of Higher Education
InventorsMohamed B. Ezzelarab, Angus W. Thomson
Life of this patent
Estimating the term…
Abstract
Pre-existing alloreactive memory T cells are a major barrier to the induction of allograft tolerance in organ transplant recipients. The use of Eomesodermin (Eomes) expression in memory T cells to determine the risk of allograft rejection in a subject is described. Also described is the use of Eomes expression in memory T cells of transplant recipients to modify immunosuppressive therapy.
Claims20
A method of determining the risk of allograft rejection in a subject, comprising:
- isolating memory T cells from a peripheral blood mononuclear cell (PBMC) sample obtained from the subject;
- measuring expression of Eomesodermin (Eomes) in the memory T cells, wherein expression of Eomes is measured in the memory T cells after exposure of the memory T cells to donor-derived cells ex vivo;
- and
- detecting an increase in expression of Eomes in the memory T cells exposed to donor-derived cells compared to expression of Eomes in control memory T cells, thereby determining an increased risk for allograft rejection in the subject.
1 more element
A method of treating a subject who is at risk of allograft rejection, comprising:
- administering an immunosuppressive therapy to the subject;
- isolating memory T cells from a peripheral blood mononuclear cell (PBMC) sample obtained from the subject;
- measuring expression of Eomesodermin (Eomes) in the memory T cells, wherein expression of Eomes is measured in the memory T cells after exposure of the memory T cells to donor-derived cells ex vivo;
- and
- detecting an increase in expression of Eomes in the memory T cells exposed to donor-derived cells compared to expression of Eomes in control memory T cells, thereby determining an increased risk for allograft rejection in the subject;
- and
- modifying the immunosuppressive therapy administered to the subject.
4 more elements
Dependencies are inferred from the claim text.