Algorithms for Disease Diagnostics

A method, comprising:

• (a) obtaining a sample from a subject, wherein said subject is suspected of having lung cancer based at least in part on an imaging analysis of said subject showing a suspicious lesion;
• (b) assaying gene expression levels in said sample;
• and
• (c) processing said gene expression levels to determine that said suspicious lesion is associated with or is likely to be associated with said lung cancer in said subject;
• wherein said sample comprises respiratory epithelial cells from a mouth, nose, pharynx, trachea, or bronchi of said subject.