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US 2021/0262013 A1Published application
Nucleic acid cleaving enzyme-based biosensor and methods of use thereof
AssigneeMcMaster University
InventorsCarlos Filipe, Yingfu Li, Sahar Esmaeili Samani, Dingran Chang, Erin M. McConnell, Meghan Rothenbroker
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Abstract
This disclosure relates to biosensors, and in particular, biosensors based on nucleic acid cleaving enzymes such as ribonucleotide-cleaving DNAzymes for the detection of analytes, and methods of use.
Claims19
A biosensor for the detection of an analyte comprising:
- a) i) a nucleic acid substrate coupled to a first support and a nucleic acid cleaving enzyme;
- or
- ii) a nucleic acid cleaving enzyme comprising a nucleic acid substrate coupled to a first support;
- and
- b) a nucleic acid binding molecule coupled to a microzone on a second support;
- wherein the nucleic acid substrate comprises a detection moiety;
- wherein the nucleic acid enzyme is capable of cleaving the nucleic acid substrate;
- wherein the nucleic acid cleaving enzyme is activated upon contact with the analyte, thereby cleaving the nucleic acid substrate to release a cleavage product comprising the detection moiety;
- and
- wherein the nucleic acid binding molecule binds to the cleavage product.
7 more elements
Dependencies are inferred from the claim text.