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US 2021/0261597 A1Published application
Compositions and methods for synthesizing 5'-capped rnas
AssigneeTriLink BioTechnologies, LLC
InventorsRichard I. HOGREFE, Alexandre LEBEDEV, Anton P. MCCAFFREY, Dongwon SHIN
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Abstract
Provided herein are methods and compositions for synthesizing 5′Capped RNAs wherein the initiating capped oligonucleotide primers have the general form m7 Gppp[N2′Ome]n[N]m wherein m7G is N7-methylated guanosine or any guanosine analog, N is any natural, modified or unnatural nucleoside, “n” can be any integer from 0 to 4 and “m” can be an integer from 1 to 9.
Claims1
An initiating capped oligonucleotide primer comprising the following structure:
- wherein:
- each of B1 through B10 is independently a natural, modified or unnatural nucleoside base;
- M is 0 or 1;
- L is 0 or 1;
- q1 is 1 and each of q2 through q9 is independently 0 or 1;
- R1 is H or methyl;
- R2 and R3 are independently H, OH, alkyl, O-alkyl, halogen, a linker or a detectible marker;
- each of X1 through X13 is independently O or S;
- each of Y1 through Y13 is independently OH, SH, BH3, aryl, alkyl, O-alkyl or O-aryl;
- each of Z1 through Z22 is independently O, S, NH, CH2, C(halogen)2 or CH(halogen);
- and
- each of R4 through R12 are independently H, OH, OMe or a detectable marker.
9 more elements
Dependencies are inferred from the claim text.