Novel methods for the production of pharmaceutical agents

The method according to any of the preceding claims, wherein said liquid is a blood sample, preferably a whole blood sample or a whole blood sample from which erythrocytes have been depleted.

The method according to any of the preceding claims, wherein said agitation means is selected from

• a wheel-shaped or propeller-shaped device for rotating the container around an axis thereof, wherein preferably the device has a rotation axis that is perpendicular to the direction of gravity or tilted by an angle of up to 90 degrees, and
• a shaking device, which is preferably adapted to carry out a circular or translational motion.

The method according to any of the preceding claims, wherein said incubation is performed under one or more of the following conditions:

• for an incubation time of 15 min to 24 hours, 1 hour to 6 hours, 1 hour to 5 hours, 1.5 hours to 24 hours, 2 hours to less than 24 hours, 2 hours to 5 hours, at least 3 hours, 3 hours to 5 hours, 3 hours to less than 24 hours, 3 hours to 20 hours, 3 hours to 16 hours, 4 hours to 12 hours, at least 5 hours, 5 hours to 10 hours, 5 hours to 7 hours, up to 6 hours, at least 6 hours or at least 7 hours,
• at a temperature from 30° C. to 40° C., preferably at 37° C., or
• in the absence or presence of an added anticoagulant.

The method according to any of the preceding claims, wherein said cellular constituents of blood comprise platelets and step (e) is a step of, after said incubation time has passed, obtaining said pharmaceutical composition by degranulating said platelets by adding calcium ions and/or thrombin, and thereafter collecting said liquid.

A pharmaceutical composition produced by the method of any of the preceding claims.

A kit comprising:

• a container having an internal surface for being contacted with a liquid collected from a mammal, which liquid comprises cellular constituents of blood,
• an agitation means for agitating said liquid at least once during an incubation time in which said liquid is incubated in contact with said internal surface and preferably
• a means for application of a pharmaceutical composition to a mammal, wherein said pharmaceutical composition is obtained by removing part of or the entirety of said cellular constituents from said liquid and collecting the remainder after said incubation time.