A method for producing a cheese with reduced amount of galactose
AssigneeChr. Hansen A/S
InventorsThomas JANZEN, Ditte Ellegaard CHRISTIANSEN, Veronique JACTAT, Kasper ROSENKVIST, Charlotte Elisabeth Grüner SCHÖLLER
Life of this patent
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Abstract
A method for producing a cheese with reduced amount of galactose comprising inoculating milk with Streptococcus thermophilus Gal(+) bacteria and Lactobacillus Gal(+) Lac(−) bacteria.
Claims15
A method for producing a cheese with reduced amount of galactose comprising following steps:
- (a):
- inoculating milk with:
- (I):
- Streptococcus thermophilus (ST) bacteria, characterized by that the ST bacteria are able to reduce by at least 10% the amounts of excreted galactose in milk as compared to reference ST CHCC4323 (DSM 32826) bacteria (herein termed “ST Gal(+) bacteria”);
- wherein the comparative test is performed by that the ST bacteria are inoculated in skim cow milk 1% from overnight cultures and incubated for 18 hours at 37° C. and at the end of the fermentation samples are taken to measure galactose content in the fermented milk and thereby measure the reduction of excreted galactose compared to the reference CHCC4323;
- and
- (II):
- Lactobacillus (Lb.) bacteria, characterized by that the Lb. bacteria are able to ferment galactose but not able to ferment lactose (herein termed “Lb. Gal(+) Lac(−) bacteria”);
- wherein “able to ferment galactose” is measured by that the Lb. bacteria are inoculated in MRS (De Man, Rogosa and Sharpe) medium containing 1% galactose as sole carbohydrate 1% from overnight cultures and incubated for 24 hours at 37° C. and at the end of the fermentation samples are taken to measure pH and if the pH has decreased by at least 0.5 then is the Lb. “able to ferment galactose” (herein termed “LB. Gal(+) bacteria”);
- and wherein “not able to ferment lactose” is measured by that the Lb. bacteria are inoculated in MRS medium containing 1% lactose as sole carbohydrate 1% from overnight cultures and incubated for 18 hours at 37° C. and at the end of the fermentation samples are taken to measure pH and if the pH has not decreased by more than 1.0 then is the Lb. “not able to ferment lactose” (herein termed “LB. Lac(−) bacteria”);
- and
- (b):
- fermenting the milk with the bacteria of (a);
- and
- (c):
- making further adequate steps to finally end up with the produced cheese with reduced amount of galactose.
The method of any of the preceding claims, wherein the cheese is a cheese used in a process (e.g. for making pizza) involving a heating step to a temperature above 70° C. and wherein the cheese is a pasta filata cheese, a pizza cheese or a mozzarella cheese.
The method of any of the preceding claims, wherein the Streptococcus thermophilus (ST) bacteria cell is at least one cell selected from the group consisting of:
- (a) a Streptococcus thermophilus cell CHCC19097 deposited with registration number DSM 32594;
- (b) a Streptococcus thermophilus cell CHCC19100 deposited with registration number DSM 32595;
- (c) a Streptococcus thermophilus cell CHCC27912 deposited with registration number DSM 32596;
- (d) a Streptococcus thermophilus cell CHCC29526 deposited with registration number DSM 32597;
- (e) a Streptococcus thermophilus cell CHCC29530 deposited with registration number DSM 32598;
- and
- (f):
- a Streptococcus thermophilus cell CHCC14994 deposited with registration number DSM 25838.
The method of any of the preceding claims, wherein the Lactobacillus (Lb.) bacteria cell is at least one cell selected from the group consisting of:
- Lactobacillus rhamnosus CHCC3402, deposit number ATCC53103;
- Lactobacillus paracasei CHCC6272, deposit number DSM 16572;
- and
- Lactobacillus delbrueckii ssp. lactis CHCC27906, deposit number DSM 32831.
A Lactobacillus delbrueckii subsp. lactis cell CHCC27906 deposited with registration number DSM 32831.
Dependencies are inferred from the claim text.