Back to results
US 2021/0259218 A1Published application
METHODS OF GENETIC MEDIATED ENGINEERING OF RNAi MODELS
AssigneeMirimus, Inc.
InventorPrem PREMSRIRUT
Life of this patent
Estimating the term…
Abstract
Provided herein are systems and methods for Inducible and conditional CRISPR/Cas9 and RNAi. From animal model creation and the efficiency of CRISPR-based targeting, the present invention comprises developing RNAi models that enable inducible and reversible gene silencing to simulate new therapeutic regimes.
Claims14
A method of establishing founder knock-in strains comprising:
- a. creating a founder strain with a nucleotide sequence comprising a promoterand a miRNA backbone;
- and
- b. using the founder strain to knockin a variable shRNA sequence for a subsequent strain to be produced.
A system for creating of RNAi rats comprising:
- a. Generating a first founder strain, wherein the first founder strain includes a nucleotide sequence consisting of a promoter and a backbone miRNA system;
- and
- b. Using the first founder strain and incorporating an shRNA targeting sequence.
Dependencies are inferred from the claim text.