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US 2021/0239718 A1Published application
Methods for detecting vitamin c by mass spectrometry
AssigneeQuest Diagnostics Investments Incorporated
InventorsQibo Jiang, Richard E. Reitz, Sum Chan
Life of this patent
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Abstract
Provided are methods for determining the amount of vitamin C in a sample using mass spectrometry. The methods generally involve ionizing vitamin C in a sample and detecting and quantifying the amount of the ion to determine the amount of vitamin C in the sample.
Claims7
A method for determining an amount of ascorbic acid (vitamin C) in a sample, the method comprising:
- (i) extracting vitamin C by high turbulence liquid chromatography;
- (ii) ionizing vitamin C in the sample by atmospheric pressure chemical ionization (APCI) in negative ion mode to generate at least one vitamin C ion with a mass/charge ratio of 114.85±0.50 and/or 86.85±0.50 that is detectable by mass spectrometry;
- (iii) determining the amount of the at least one said vitamin C ion by tandem mass spectrometry;
- wherein the amount of vitamin C in the sample is determined from the amount of vitamin C ion(s) from step (iii).
1 more element
A method for determining an amount of ascorbic acid (vitamin C) in a sample, the method comprising:
- (i) purifying vitamin C in the test sample by high turbulence liquid chromatography (HTLC) followed by high performance liquid chromatography (HPLC);
- (ii) ionizing vitamin C in the sample by atmospheric pressure chemical ionization (APCI) in negative ion mode to generate at least one vitamin C ion with a mass/charge ratio of 114.85±0.50 and/or 86.85±0.50 that is detectable by mass spectrometry;
- (iii) determining the amount of the at least one said C ion by tandem mass spectrometry;
- wherein the amount of vitamin C in the sample is determined from the amount of vitamin C ion(s) from step (iii).
1 more element
Dependencies are inferred from the claim text.