Immunoreactive polypeptides
AssigneeResearch Development Foundation
InventorsJere MCBRIDE, David H. Walker
Life of this patent
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Abstract
Methods and compositions for diagnosing and vaccinating against Ehrlichia chaffeensis are provided.
Claims86
A method of detecting antibodies that specifically bind an Ehrlichia organism in a test sample, comprising:
- (a) contacting an isolated polypeptide of Table 1, Table 2, or Table 3 or a polypeptide having at least 95% sequence identity thereto, with the test sample, under conditions that allow peptide-antibody complexes to form;
- (b) detecting the peptide-antibody complexes;
- wherein the detection of the peptide-antibody complexes is an indication that antibodies specific for an Ehrlichia organism are present in the test sample, and wherein the absence of the peptide-antibody complexes is an indication that antibodies specific an Ehrlichia organism are not present in the test sample.
A method of identifying an Ehrlichia infection in a mammalian subject comprising:
- (a) contacting a biological sample from the subject with an isolated polypeptide of Table 1, Table 2, or Table 3 under conditions that allow peptide-antibody complexes to form;
- and (b) detecting the peptide-antibody complexes;
- wherein the detection of the peptide-antibody complexes is an indication that the subject has an Ehrlichia infection.
An isolated polypeptide comprising a sequence of Table 1, Table 2, or Table 3, wherein the isolated peptide is immobilized on a surface of a support substrate.
An isolated polypeptide comprising a sequence of Tablet, Table 2, or Table 3, wherein the isolated peptide is covalently attached to a detectable label.
A method of inducing an immune response in a mammalian subject comprising administering to the subject an effective amount of a pharmaceutical preparation comprising a polypeptide of Table 1, Table 2, or Table 3.
A method of treating an Ehrlichia chaffeensis infection in a subject comprising:
- (a) contacting a biological sample from the subject with an isolated polypeptide of Table 1, Table 2, or Table 3 under conditions that allow peptide-antibody complexes to form;
- (b) detecting the peptide-antibody complexes;
- wherein the detection of the peptide-antibody complexes is an indication that the subject has an Ehrlichia chaffeensis infection;
- and
- (c) administering a therapeutic compound to treat Ehrlichia infection in the subject.
A method of detecting antibodies that specifically bind an Ehrlichia organism in a test sample, comprising:
- (a) contacting an isolated polypeptide of:
- Ecaj_0919, Ecaj_0073, Ecaj_0104, Ecaj_0663, or Ecaj_0881, more preferably Ecaj_0919, Ecaj_0073, Ecaj_0104, or Ecaj_0663, even more preferably Ecaj_0919, Ecaj_0073, Ecaj_0104, even more preferably Ecaj_0919 or Ecaj_0073;
- or a polypeptide having at least 95% sequence identity thereto, with the test sample, under conditions that allow peptide-antibody complexes to form;
- (b) detecting the peptide-antibody complexes;
- wherein the detection of the peptide-antibody complexes is an indication that antibodies specific for an Ehrlichia organism are present in the test sample, and wherein the absence of the peptide-antibody complexes is an indication that antibodies specific an Ehrlichia organism are not present in the test sample.
A method of identifying an Ehrlichia infection in a mammalian subject comprising:
- (a) contacting a biological sample from the subject with an isolated polypeptide of Ecaj_0919, Ecaj_0073, Ecaj_0104, Ecaj_0663, or Ecaj_0881, more preferably Ecaj_0919, Ecaj_0073, Ecaj_0104, or Ecaj_0663, even more preferably Ecaj_0919, Ecaj_0073, Ecaj_0104, even more preferably Ecaj_0919 or Ecaj_0073, under conditions that allow peptide-antibody complexes to form;
- and (b) detecting the peptide-antibody complexes;
- wherein the detection of the peptide-antibody complexes is an indication that the subject has an Ehrlichia infection.
An isolated polypeptide comprising a sequence of Ecaj_0919, Ecaj_0073, Ecaj_0104, Ecaj_0663, or Ecaj_0881, more preferably Ecaj_0919, Ecaj_0073, Ecaj_0104, or Ecaj_0663, even more preferably Ecaj_0919, Ecaj_0073, Ecaj_0104, even more preferably Ecaj_0919 or Ecaj_0073, wherein the isolated peptide is immobilized on a surface of a support substrate.
An isolated polypeptide comprising a sequence of Ecaj_0919, Ecaj_0073, Ecaj_0104, Ecaj_0663, or Ecaj_0881, more preferably Ecaj_0919, Ecaj_0073, Ecaj_0104, or Ecaj_0663, even more preferably Ecaj_0919, Ecaj_0073, Ecaj_0104, even more preferably Ecaj_0919 or Ecaj_0073, wherein the isolated peptide is covalently attached to a detectable label.
A method of inducing an immune response in a mammalian subject comprising administering to the subject an effective amount of a pharmaceutical preparation comprising a polypeptide of Ecaj_0919, Ecaj_0073, Ecaj_0104, Ecaj_0663, or Ecaj_0881, more preferably Ecaj_0919, Ecaj_0073, Ecaj_0104, or Ecaj_0663, even more preferably Ecaj_0919, Ecaj_0073, Ecaj_0104, even more preferably Ecaj_0919 or Ecaj_0073.
A method of treating an Ehrlichia canis infection in a mammalian subject comprising:
- (a) contacting a biological sample from the subject with an isolated polypeptide of Ecaj_0919, Ecaj_0073, Ecaj_0104, Ecaj_0663, or Ecaj_0881, more preferably Ecaj_0919, Ecaj_0073, Ecaj_0104, or Ecaj_0663, even more preferably Ecaj_0919, Ecaj_0073, Ecaj_0104, even more preferably Ecaj_0919 or Ecaj_0073, under conditions that allow peptide-antibody complexes to form;
- (b) detecting the peptide-antibody complexes;
- wherein the detection of the peptide-antibody complexes is an indication that the subject has an Ehrlichia canis infection;
- and
- (c) administering a therapeutic compound to treat Ehrlichia infection in the subject.
Dependencies are inferred from the claim text.