Implement for inspection, inspecting device and inspecting method

An inspecting method using the implement for inspection for measuring a concentration of a test substance,

• the implement for inspection comprising:
• a compound for reacting with the test substance to form a granular substance or a compound which is for being bound to the test substance and is a granular substance;
• and
• a wall portion having a periodic structure on a surface of the wall portion,
• wherein the periodic structure generates a peak of reflected light in a wavelength region of a photonic band gap when light is applied to the periodic structure,
• the inspecting method comprising steps (1) to (4) below:
• a step (1) of applying light to the periodic structure to measure reference light intensity in a state where the test substance is not introduced into the implement for inspection;
• a step (2) of introducing the test substance into the implement for inspection and depositing the granular substance formed by reacting the test substance with the compound or a granular substance in which the test substance is bound to the compound on the periodic structure,
• a step (3) of applying light to the periodic structure to measure evaluation light intensity in a state where the granular substance is deposited on the periodic structure;
• and
• a step (4) of acquiring a relative value of the reference light intensity and the evaluation light intensity, wherein
• when the relative value obtained when a sample in which a concentration of the test substance is known is introduced into the implement for inspection is compared to the relative value obtained when a sample in which a concentration of the test substance is unknown is introduced into the implement for inspection, the concentration of the test substance in the sample in which the concentration of the test substance is unknown is determined.